解放军医学杂志2017，Vol.42Issue(3)：217-223,7.DOI:10.11855/j.issn.0577-7402.2017.03.08
潮汕汉族群体27个Y-STR基因座的单倍型及遗传关系分析
Haplotype and genetic relationship of 27 Y-STR loci in Han population of Chaoshan area of China
摘要
Abstract
Objective To investigate the genetic polymorphisms of 27 Y-chromosomal short tandem repeats (Y-STR) loci included in Yfiler(R) Plus kit in Han population of Chaoshan area,and explore the population genetic relationships and evaluate its application value on forensic medicine.Methods By detecting 795 unrelated Chaoshan Han males with Yfiler(R) Plus kit,haplotype frequencies and population genetics parameters of the 27 Y-STR loci were statistically analyzed and compared with available data of other populations from different races and regions for analyzing the genetic distance and clustering relation of Chaoshan Han population.Results Seven hundred and eighty-seven different haplotypes were observed in 795 unrelated male individuals,of which 779 haplotypes were unique,and 8 haplotypes occurred twice.The haplotype diversity (HD) was 0.999975 with discriminative capacity (DC) of 98.99％.The gene diversity (GD) at the 27 Y-STR loci ranged from 0.3637(DYS391) to 0.9559(DYS385a/b).Comparing with Asian reference populations,the genetic distance (Rst) between Chaoshan Han and Guangdong Han was the smallest (0.0036),while it was relatively larger between Chaoshan Han and Gansu Tibetan population (0.0935).The multi-dimensional scaling (MDS) plot based on Rst values was similar to the results of clustering analysis.Conclusion Multiplex detection of the 27 Y-STR loci reveals a highly polymorphic genetic distribution in Chaoshan Han population,which demonstrates the important significance of Yfiler(R) Plus kit for establishing a Y-STR database,studying population genetics,and for good practice in forensic medicine.关键词
Y染色体短串联重复序列/单倍型/多维尺度分析/汉族Key words
Y-chromosomal short tandem repeats(Y-STR)/haplotype/multi-dimensional scaling/Han nationality分类
医药卫生引用本文复制引用
田庆花,王俊方,张晶,刘亚举,石美森..潮汕汉族群体27个Y-STR基因座的单倍型及遗传关系分析[J].解放军医学杂志,2017,42(3):217-223,7.基金项目
国家自然科学基金(81671874) （81671874）
中国政法大学研究生创新实践项目(2015SSCX160)This work was supported by the National Natural Science Foundation of China(81671874),and the Innovation Fund Project of Graduate Student of China University of Political Science and Law(2015SSCX160) （2015SSCX160）
