吉林大学学报（理学版）2017，Vol.55Issue(2)：430-434,5.DOI:10.13413/j.cnki.jdxblxb.2017.02.41
甲基丙烯酸-8-羟基喹啉-Eu(Ⅲ)配合物与鲱鱼精DNA的作用机制
Mechanism of Interaction between Eu(Ⅲ) Complex with Methacrylic Acid-8-hydroxyquinoline and Herring Sperm DNA
摘要
Abstract
We studied the mechanism of interaction and binding constant between Eu (Ⅲ)complex with methaerylic acid-8-hydroxyquinoline (Eu (MA )2 (hq )) and herring sperm DNA by using spectroscopic methods,viscosity and thermal denaturation method of DNA.Results show that the absorption peak has obvious hypochromic effect,but red shift phenomenon of peak is not obvious after the complex adding herring sperm DNA.The complex can quench the fluorescence of neutral red-DNA system.The binding ratio of n(Eu(MA)2 (hq)):n(DNA)is 1 ,and the binding constants are K20= 5.91 × 103 L/mol,and K35=7.70 × 103 L/mol.The thermodynamic functions between complex and herring sperm DNA are Δr H?m=1.34×103 J/mol,Δr G?m=-2.12×104 J/mol,andΔr S?m=76.41 J/(mol·K).The relative viscosity and melting point of herring sperm DNA are increased with the addition of complex,and the interaction mode of the Eu(MA)2 (hq)with herring sperm DNA belongs to intercalation.关键词
甲基丙烯酸-8-羟基喹啉-Eu(Ⅲ)/配合物/鲱鱼精/DNA/相互作用Key words
methacrylic acid-8-hydroxyquinoline-Eu(Ⅲ)/complex/herring sperm/DNA/interaction分类
化学化工引用本文复制引用
李小芳,冯小强..甲基丙烯酸-8-羟基喹啉-Eu(Ⅲ)配合物与鲱鱼精DNA的作用机制[J].吉林大学学报（理学版）,2017,55(2):430-434,5.基金项目
甘肃省青年科技基金(批准号:1606RJYE247). （批准号:1606RJYE247）
