吉林大学学报（理学版）2017，Vol.55Issue(2)：451-457,7.DOI:10.13413/j.cnki.jdxblxb.2017.02.44
黑麦草幼苗在冻融及NaHCO3复合胁迫下的生理响应
Physiological Response of Ryegrass Seedlings under Freezing-Thawing and Combined with NaHCO3 Stress
摘要
Abstract
Using Dongmu 70 ryegrass seedlings as experimental material, we investigated its physiological response mechanism under the single artificial simulated freezing-thawing stress (10,5, 0,-5,0,5,10 ℃)and combined with NaHCO3 stress.The results show that the content of proline and malondialdehyde (MDA)and the activity of catalase (CAT)and superoxide dismutase (SOD)in the leaves of ryegrass seedlings increased firstly and then decreased significantly under a single freezing-thawing stress,the activity of peroxidase (POD)increased firstly and then decreased slowly, except for CAT,other physiological indexes reach the maximum value at -5 ℃,which compared with other temperatures there are significant differences (P<0.05).Under the condition of combined stress treatment,physiological indexes show the rule of combined stress>single stress>control.The mass ratio of protein in the leaves of ryegrass seedlings raech the maximum value at 0 ℃,and combined stress and single freezing-thawing group are higher than those in control group.Thus,the ryegrass seedling under the single freezing-thawing stress and combined with NaHCO3 stress can rapidly improve the activities of antioxidant enzymes and the mass ratio of osmotic adjustment substances to alleviate damage caused by stress,and the effects of combined stress on seedlings are greater than the single stress.关键词
冻融胁迫/NaHCO3胁迫/黑麦草/生理响应Key words
freezing-thawing stress/NaHCO3 stress/ryegrass/physiological response分类
农业科技引用本文复制引用
巩泽,包国章,李艳芳,刘滨硕..黑麦草幼苗在冻融及NaHCO3复合胁迫下的生理响应[J].吉林大学学报（理学版）,2017,55(2):451-457,7.基金项目
国家自然科学基金(批准号:31070286 （）
31270367)和吉林省自然科学基金(批准号:20150101089JC). （批准号:20150101089JC）
