吉林农业大学学报2016，Vol.38Issue(6)：675-680,6.DOI:10.13327/j.jjlau.2016.3049
秋水仙素离体诱导分蘖洋葱茎尖多倍体的研究
In Vitro Induction of Polyploid by Colchicine in Shoot Tips of Tillered Onion
摘要
Abstract
In this experiment,shoot tip explants of tillered onion strain Z-018 were used as materials,which were inoculated on solid medium added with inducer in vitro induced,and chromosome of shoot tips of tillered onion was induced at different concentration and treatment duration,in order to obtain ploidy variation of tillered onion materials.The results showed that when colchicine concentration was 0.01％ with the treatment of 4 days,polyploid induction rate was the highest,up to 42.22％.Compared with normal diploid plants,variant plants showed slow growth,leaf,false stem and roots were thicker,stomatal guard cells of leaves became larger,and density decreased.Further identification of root tip chromosome number and flow cytometry analysis of DNA content were conducted,and in 149 strains of variant strains,117 strains were obtained,of which 81 were tetraploid materials,accounting for 54.36％ of the total variation.关键词
分蘖洋葱/秋水仙素/多倍体诱导/染色体数/DNA含量Key words
tillered onion/colchicine/polyploidy induction/chromosome number/DNA content分类
农业科技引用本文复制引用
宋策,陈典,梁誉,王勇..秋水仙素离体诱导分蘖洋葱茎尖多倍体的研究[J].吉林农业大学学报,2016,38(6):675-680,6.基金项目
黑龙江省自然科学基金项目(C2015017),黑龙江省科技特派员项目(GC13B09) （C2015017）
