吉林农业大学学报2016，Vol.38Issue(6)：733-738,6.DOI:10.13327/j.jjlau.2016.3193
褪黑素对热应激猪精子质量的影响
Effect of Melatonin on Sperm Quality of Heat Stressed Boar
摘要
Abstract
The aim of this study is to investigate the alleviation effect of the melatonin (MLT) on influence of heat stress on sperm quality and its mechanism.We firstly detected the existence and distribution of MLT receptors MT1 and MT2 in boar sperm via immunofluorescence analysis.Then we divided the diluted fresh boar sperm into six groups:control group (C),MLT treated group (M),heat stressed group (H),co-treated with heat stress and MLT group (H+M),co-treated with heat stress and luzindole group (H+L),and co-treated with heat stress,luzindole and MLT group (H+L +M).Then we detected sperm viability,motility,mitochondrial activity,calcium concentration and acrosome integrity.The results showed that heat stress significantly reduced the quality of sperm,and MLT did not significantly (P＞0.05) improve the sperm viability of the heat stressed boar,but significantly enhanced motility,mitochondrial activity,calcium level and acrosomal integrity.Moreover,luzindole treatment could attenuate these effects of MLT to some degree via blocking its receptor.These results suggested that heat stress impaired boar sperm quality and administration of MLT to heat stressed sperm alleviate the adverse effect of heat stress via receptor mediated and non-receptor mediated action.关键词
精子质量/褪黑素/热应激/猪Key words
sperm quality/MLT/heat stress/boar分类
农业科技引用本文复制引用
朱彦宾..褪黑素对热应激猪精子质量的影响[J].吉林农业大学学报,2016,38(6):733-738,6.基金项目
西藏自治区财政专项[藏财预指(2016)01号] （2016）
