吉林农业大学学报2017，Vol.39Issue(1)：42-48,7.DOI:10.13327/j.jjlau.2017.2706
不同产地黄芩ITS2和psbA-trnH条形码序列分析
Sequence Analysis of ITS2 and psbA-trnH DNA Barcode of Wild Populations of Scutellaria baicalensis Georgi from Different Producing Areas
摘要
Abstract
The standardized and automated identification for Chinese medicinal materials is important for the safe use and international trade of medicinal plants,including the identification for genuine or non-genuine medicinal plants.Scutellaria baicalensis Georgi is a perennial herb of the family Lamiaceae.Root of the herb is one of the most important heat-clearing and dehumidifying drugs in traditional Chinese medicine.The wild populations of S.baicalensis are widely distributed from Northeast to Northwest China.The technology of DNA bar-coding was used to identify different germplasms of S.baicalensis.The ITS2 and psbA-trnH were selected as DNA barcode because of their quick evolutionary rate in the experiment.The genomic DNAs were extracted from wild population of S.baicalensis at ten districts,six provinces.The segments of two barcodes were amplified and aligned.The aligned sequences of ITS2 and psbA-trnH were 232 and 318 base pairs in length.The results showed that only one site of "G" insertion/deletion was found,which was located at the 53th base in the ITS2 alignment.The site was detected in the samples of Xianghai,Qianguo,Zhenlai,Taonan,Changchun of Jilin province,but not in other samples from other five provinces.The site can be considered as reference to identify the wild S.baicalensis in Jilin province.关键词
黄芩/ITS2/psbA-trnH/DNA条形码Key words
Scutellaria baicalensis Georgi/ITS2/psbA-trnH/DNA barcode分类
农业科技引用本文复制引用
吕泽良,张永刚,韩梅,刘翠晶..不同产地黄芩ITS2和psbA-trnH条形码序列分析[J].吉林农业大学学报,2017,39(1):42-48,7.基金项目
国家自然科学基金青年科学基金(31200178),吉林省博士后科研项目(RB201351) （31200178）
