吉林中医药2017，Vol.37Issue(2)：159-161,3.DOI:10.13463/j.cnki.jlzyy.2017.02.015
鹿茸玛咖配伍抑制乙酰胆碱酯酶活性部位的HPLC-DAD-ESI-MS/MS分析研究
Analysis of cornu cervi pantotrichum and maca inhibition of HPLC-DAD-ESI-MS / MS of acetylcholinesterase active site
摘要
Abstract
Objective To study the inhibitory effect of extracts of Acanthopanax on the extraction site in vitro and to analyze the chemical components of the active fraction.Methods In order to study the inhibitory effect of Acetylcholinesterase (AChE) activity in vitro on the extraction site of extracts from different extracts of the extracts from pilose antler and mako,the inhibitory effects of extracts on the acetylcholinesterase in vitro were studied by high performance liquid chromatography-Mass Spectrometry (LC-MS) was used to analyze the chemical constituents of AChE.Results Acetylcholinesterase activity was inhibited by ethyl acetate extract of pilose antler,and the potential effect of anti-Alzheimer's disease was found.After analysis and analysis,six malicamides in the ethyl acetate extraction site were deduced by LC / structure of two deer antler oligopeptides.Conclusion It is cornu cervi pantotrichum and maca ethyl acetate extraction site that are the active fraction of acetylcholinesterase inhibitor in vitro,in which components the mafionamides and velvet oligopeptides are the main material basis for the inhibition of acetylcholinesterase in vitro.关键词
鹿茸/玛咖/乙酸乙酯萃取部位/乙酰胆碱酯酶/高效液相色谱-质谱联用法Key words
cornu cervi pantotrichum/maca/ethyl acetate extraction/acetylcholinesterase/HPLC-MS/MS分类
医药卫生引用本文复制引用
孟令文,杨银平,梁新合,闫伟,张辉,孙佳明..鹿茸玛咖配伍抑制乙酰胆碱酯酶活性部位的HPLC-DAD-ESI-MS/MS分析研究[J].吉林中医药,2017,37(2):159-161,3.基金项目
国家自然基金课题(编号:81373936、31570347) （编号:81373936、31570347）
吉林省卫生厅科研课题(编号:2013Z041) （编号:2013Z041）
吉林省科技发展计划项目(编号:20140203015YY) （编号:20140203015YY）
吉林省教育厅“十二五”科学技术研究项目(吉教科合字[2014]第73号). （吉教科合字[2014]第73号）
