军事医学2017，Vol.41Issue(3)：161-164,4.DOI:10.7644/j.issn.1674-9960.2017.03.001
线粒体呼吸链在高压氧诱导HUVEC活性氧产生中的作用
The role of mitochondrial respiratory chain in hyperbaric oxygen induced reactive oxygen species in HUVEC
摘要
Abstract
Objective To investigate the role of mitochondrial respiratory chain (MRC) in induction of reactive oxygen species (ROS) by hyperbaric oxygen (HBO).Methods Superoxide anion (O·-2) specific fluorescence probe DHE and mitochondrial O·-2 probe Mito-SOX were used to label ROS in human umbilical vein endothelium cells(HUVEC) microscopically after HBO exposure.Results After HBO exposure, O·-2 increased (31±8)% and (137±19) % in whole cells and in mitochondria, respectively (P<0.01).These increments were suppressed by MRC complex Ⅱ inhibitor TTFA for (55±11)% in whole cells (P<0.05)and (61±8) % in mitochondria(P<0.01).Conclusion MRC may be the main source of ROS induced by HBO in HUVECs.关键词
高压氧/减压病/线粒体呼吸链/活性氧/人脐静脉内皮细胞Key words
hyperbaric oxygen/decompression sickness/mitochondrial respiratory chain/reactive oxygen species/human umbilical vein endothelium cells(HUVEC)分类
医药卫生引用本文复制引用
周全,黄国阳,徐伟刚..线粒体呼吸链在高压氧诱导HUVEC活性氧产生中的作用[J].军事医学,2017,41(3):161-164,4.基金项目
国家自然科学基金资助项目(81171873) （81171873）
