军事医学2017，Vol.41Issue(3)：169-173,177,6.DOI:10.7644/j.issn.1674-9960.2017.03.003
PIF1解旋酶在电离辐射诱发的细胞周期阻滞中的作用
Roles of PIF1 helicase in cell cycle arrest induced by ionizing radiation
摘要
Abstract
Objective To observe the effect of PIF1 knockdown on cell growth and cell cycle arrest induced by ionizing radiation.Methods HeLa cell lines that consistently down-regulated PIF1 were prepared by the lentivirus granules interfering technology and confirmed by real-time PCR and Western blotting.The effect of down-regulation of PIF1 on cell growth and cell cycle arrest induced by ionizing radiation was evaluated by cell counting and flow cytometry.Results HeLa cell lines consistently down-regulating PIF1 were established.The growth of HeLa that down-regulated PIF1 was inhibited greatly after 4 Gy of γ-ray irradiation.There was little cell proliferation until the 5th day post 4 Gy γ-ray.Moreover, the S phase block and G2/M phase block of PIF1 knock-downed cell lines were significantly delayed after 8 Gy γ-ray irradiation.Conclusion Knockdown of PIF1 can significantly enhance the radiation sensitivity and delayes the S phase block and G 2 /M phase block induced by ionizing radiation.关键词
PIF1解旋酶/辐射,电离/辐射敏感性/细胞周期阻滞/DNA损伤Key words
PIF1 helicase/radiation/ionizing/radiation sensitivity/cell cycle arrest/DNA damage分类
生物科学引用本文复制引用
李忠秋,朱茂祥,顾永清,李超,赵德根,杨洋,李雪萍,曾妍,潘秀颉,杨陟华,周平坤..PIF1解旋酶在电离辐射诱发的细胞周期阻滞中的作用[J].军事医学,2017,41(3):169-173,177,6.基金项目
国家自然科学基金资助项目(31470827,81472910,81560473,31270894) （31470827,81472910,81560473,31270894）
