军事医学2017，Vol.41Issue(3)：174-177,4.DOI:10.7644/j.issn.1674-9960.2017.03.004
连续睡眠剥夺对大鼠肝生物钟基因及肝损伤标志物表达水平的影响
Effect of continuous sleep deprivation on liver circadian clock gene expression and alteration of liver bio-marker levels in rats
摘要
Abstract
Objective To investigate the effect of 36 h continuous sleep deprivation(SD) on circadian clock gene expression in the rat liver and kidney and the alteration of urine biomarker levels.Methods Twelve rats were randomly divided into control group and SD group.An SD device was used to deprive the rats of sleep.After 36 h continuous SD, the abdominal cavity was exposed to obtain livers and kidneys, and RT-PCR and Western blotting were used to detect expression of clock genes.Then,the pelvic cavity was exposed to obtain urine, and the changes in bio-marker total bile acids(TBA) were tested with ELISA.Results Compared with the control group, the mRNA level of liver clock and bmal1 was obviously reduced in the SD-treated rats (P<0.05).However, no obvious change was found in the samples from the kidney.Sharp down-regulation of CLOCK and BMAL1 protein expression was also observed in the rat liver after SD treatment.Urine TBA content in SD treated rats was raised obviously (P<0.001), compared with control.Conclusion Thirty-six hours of continuous SD could result in deregulation of circadian clock gene and cholesterol metabolism disorder in the rat liver.TBA might be used as a noninvasive biomarker of liver injury under SD stress conditions.关键词
连续睡眠剥夺/生物钟基因/昼夜节律/肝损伤标志物Key words
continuous sleep deprivation/circadian clock genes/circadian rhythm/liver injury marker分类
医药卫生引用本文复制引用
顾晔,邢陈,宋伦,黎卫平..连续睡眠剥夺对大鼠肝生物钟基因及肝损伤标志物表达水平的影响[J].军事医学,2017,41(3):174-177,4.基金项目
军队"十二五"重大资助项目(AWS14J011) （AWS14J011）
北京市自然科学基金青年基金资助项目(7164287) （7164287）
细胞应激生物学国家重点实验室(厦门大学)开放基金资助项目(SKLCSB2016KF010) （厦门大学）
