解放军医学院学报2016，Vol.37Issue(12)：1273-1277,1292,6.DOI:10.3969/j.issn.2095-5227.2016.12.015
miR-181a在肾透明细胞癌中的表达及对肾透明细胞癌细胞786-O功能的影响
Expression of miR-181a in human clear cell renal carcinoma and its effect on biological behavior of clear cell renal carcinoma 786-O cells
摘要
Abstract
Objective To investigate the expression of miR-181a in patients with clear cell renal carcinoma (ccRCC) and its effect on biological behaviors of human ccRCC cell line 786-O.Methods The expression of miR-181a in 42 tumor tissues and paired adjacent normal tissues of ccRCC,and ccRCC cell lines 786-O,769-P,A498,caki-1 were detected by qRT-PCR.Chemically synthesized miR-181a mimics or inhibitor were transfected into ccRCC cell line 786-O,then its proliferation was observed by MTS assay and its cell cycle and apoptosis were detected by flow cytometric analysis.Results Compared with adjacent noncancerous tissues,miR-181 a expression increased significantly in ccRCC tissues,and it was also significantly higher in cell lines 786-O,769-P,A498,caki-1 than in human kidney tubule epithelial cell (HKC).miR-181a mimics significantly promoted cell proliferation,G1/S transition in cell cycle and inhibited cell apoptosis in ccRCC cell line 786-O,however,miR-181 a inhibitor significantly inhibited cell proliferation,arrested cells in G1 phase while promoted cell apoptosis.Conclusion The miR-181a expresses highly in ccRCC and also participates in the occurrence and development of tumor,which may be a valuable prognostic predictor and a potential therapeutic target of ccRCC.关键词
miR-181a/肾透明细胞癌/786-O细胞Key words
miR-181a/clear cell renal cell carcinoma/786-O cell分类
医药卫生引用本文复制引用
雷振伟,巫胜攀,王瀚锋,张旭,张瑜,高宇,范阳,李新涛,陈路遥,张帆,陈建文,唐露..miR-181a在肾透明细胞癌中的表达及对肾透明细胞癌细胞786-O功能的影响[J].解放军医学院学报,2016,37(12):1273-1277,1292,6.基金项目
国家高技术研究发展计划(863)资助课题(2014AA020607)Supported by the National High Technology Research and Development Program of China ("863" Program)(2014AA020607) （863）
