解放军医学院学报2016，Vol.37Issue(12)：1284-1288,5.DOI:10.3969/j.issn.2095-5227.2016.12.017
慢病毒载体用于羊骨髓间充质干细胞基因表达实验
Feasibility study of lentiviral vector-mediated modification of ovine MSCs for gene expression
梁文涛 1丁毅 2王嵘 3张勇 1李则学 1梁凯 1赵云山1
作者信息
- 1. 解放军总医院普通外科研究所,北京100853
- 2. 武装警察总医院肛肠研究所,北京100039
- 3. 解放军总医院心血管外科,北京100853
- 折叠
摘要
Abstract
Objective To study the feasibility of lentiviral vector-mediated modification on ovine marrow mesenchymal stem cells (MSCs) for gene expression.Methods Ovine MSCs were transduced into lentiviral vector MSCz by ZsGreen lentiviral vector,and the effects of transduction on morphology,proliferation and differentiation potential were evaluated using flow cytometry,MTT assay and trilealinige differentiation,respectively.Results MSCz maintained the specific morphology of mesenchymal stem cells,and expressed ZsGreen proteins as detected by fluorescent microscopy.The percentage of MSCz increased with the rise of MOI based on flow cytometry analysis,and it could reach 71.2％ with MOI of 20.MTT assay showed that the proliferation ability of MSCz were same as MSCs.The multipotent ability of MSCz showed no difference in differentiation towards adipose,chondrocyte and osteoblast from MSCs.Stress by hydrogen peroxide or culture under hypoxia showed that MSCs and MSCz had the same response to these hostile environments.Conclusion Lentiviral vector is a good vector for transducing ovine MSCs for gene expression.关键词
羊骨髓间充质干细胞/慢病毒载体/转染Key words
ovine marrow mesenchymal stem cells/lentiviral vector/transfection分类
医药卫生引用本文复制引用
梁文涛,丁毅,王嵘,张勇,李则学,梁凯,赵云山..慢病毒载体用于羊骨髓间充质干细胞基因表达实验[J].解放军医学院学报,2016,37(12):1284-1288,5.
