解放军医学院学报2016，Vol.37Issue(12)：1289-1292,4.DOI:10.3969/j.issn.2095-5227.2016.12.018
miR-199-3p在兔膝骨关节炎软骨下骨中的表达及其意义
Expression of miR-199-3p in subchondral bone from rabbits with knee osteoarthritis
摘要
Abstract
Objective To detect the expression of microRNA-199-3p (miR-199-3p) in subchondral bone of rabbit model with knee osteoarthritis (OA).Methods Twenty-four New Zealand white rabbits were randomly divided into two groups:anterior cruciate ligament transaction (ACLT) group (n=12) and sham group (n=12).OA was surgically induced in the right knee of rabbits in ACLT group.The rabbit knees were evaluated at 4,8 and 12 weeks after ACLT by gross observation and histological examination.After getting the specimens,we segregated subchondral bone,and the expression of miR-199-3p was tested by qRT-PCR.Results Rabbits in ACLT group showed typical OA pathological changes after 4 weeks,such as loss of surface integrity,fissuring and mircocracks,loss of cartilage and these symptoms aggravated at 8 and 12 weeks after operation.The expression of miR-199-3p could be detected in all subchondral bone with a decreasing trend over time,and it was significantly lower in ACLT group comparing with sham group,while the expression of MMP-3 and IL-1 β were higher in ACLT group than sham group with significant difference at each time point (P ＜ 0.05).Conclusion Our findings indicate that low expression of miR-199-3p is closely related with the development and progression of OA.关键词
骨关节炎/微RNAs/软骨下骨Key words
osteoarthritis/microRNAs/subchondral bone分类
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张静,王秀丽,彭方,唐佩福..miR-199-3p在兔膝骨关节炎软骨下骨中的表达及其意义[J].解放军医学院学报,2016,37(12):1289-1292,4.
