临床输血与检验2017，Vol.19Issue(2)：137-141,5.DOI:10.3969/j.issn.1671-2587.2017.02.013
2015年广州地区无偿献血者核酸和ELISA检测结果分析
Analyses of Laboratory Detections with PCR and ELISA in Blood Donors of Guangzhou in 2015
林诗雅 1王淏 1李仲平 1梁浩坚 1谢君谋 1肖韶英 1郑优荣1
作者信息
摘要
Abstract
Objective To analyze the results of nucleic acid tests(NATs) and ELISA detections and explore the correlation of them in reducing the risk of transfusion related infections.Methods Using two kinds of ELISA reagents and ID nucleic acid detection(ID-NAT) for parallel blood screening inblood donation to detect serological markers of HBV, HCV and HIV-1 and virus nucleic acids. Samples were taken from blood bags for further identification when primary test was positive. HIV-1 RNA positive ELISA negative blood donors were followed up.Results In total of 300250 cases,0.96%(2874/300250) positive cases were detected in the center in 2015.The nucleic acid single positive rate was 0.21%(628/300250); the identification positive rate was 20.58%(129/628).127 HBVand2 HIV cases were found and no HCV was seen. Enzyme immunoassay of HBV, HCV and HIV and nucleic acid detection results discovered 2204 positive cases .78%(1829/2333) HBV ELISA double reagent positive specimens were NAT positive, and22%(504/2333) were NAT negative;66%(226/343) HCV ELISA double reagent positivespecimens were NAT positive, and34%(117/343) were NAT negative;92 cases of HIV ELISA double reagent positive samples were all NAT positive. Six percent (57/901)ELISA single reagent positive were NAT positive, and 94%(844/901)were NAT negative. The percentage of positive nucleic acid in ELISA double reagent positive was higher than single reagent positive ELISA (P<0.05).Conclusion NAT helps shorten "window period" of transfusion transmitted diseases and detect latent infection. Both NAT and ELISA play an important complementary role in reducing the risk of blood transfusion associated virus infection.关键词
核酸扩增检/隐匿性HBV感染/窗口期/无偿献血Key words
Nucleic/acid/amplification/detection/OBI/Window/period/Voluntary/blood/donation分类
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林诗雅,王淏,李仲平,梁浩坚,谢君谋,肖韶英,郑优荣..2015年广州地区无偿献血者核酸和ELISA检测结果分析[J].临床输血与检验,2017,19(2):137-141,5.
