临床与实验病理学杂志2016，Vol.32Issue(12)：1317-1322,6.DOI:10.13315/j.cnki.cjcep.2016.12.001
RNA干扰EMS1基因表达对人胃癌MGC803细胞增殖和迁移的影响
Effect of EMS1-siRNA on the proliferation and migrationof human gastric cancer cell line MGC803
摘要
Abstract
Purpose To explore the effect of EMS1-siRNA on the growth,invasion and migration of human gastric cancer cell line MGC803.Methods It used the Colony formation assay to determine the abilities of proliferation,and flow cytometry analysis to asses cell cycle distribution and apoptosis,transwell invasion and migration experiment to determine the ability of cell invasion and migration after knockdown the expression of EMS1 in MGC803.Results These results suggested that EMS1 gene down-regulated have no affect on cell cycle and cell apoptosis,but the ability of colony formation depressed and migration lowerd obviously (P ＜ 0.05).Conclusion The results shows EMS1 gene is related to proliferation and migration of tumor.关键词
RNA干扰/EMS1基因/增殖/迁移Key words
small interference RNA/EMS1 gene/proliferation/migration分类
医药卫生引用本文复制引用
侯素平,童细焱,谢海龙..RNA干扰EMS1基因表达对人胃癌MGC803细胞增殖和迁移的影响[J].临床与实验病理学杂志,2016,32(12):1317-1322,6.基金项目
国家自然科学基金(81172576)、湖南省高校创新平台开放基金(14K085)、河北省卫计委课题(ZD20140219) （81172576）
