临床与实验病理学杂志2016，Vol.32Issue(12)：1343-1347,5.DOI:10.13315/j.cnki.cjcep.2016.12.006
中国人Burkitt淋巴瘤中ID3、TCF3及MYC基因突变分析
ID3, TCF3 and MYC genes mutation analyses in Chinese Burkitt lymphoma
摘要
Abstract
Purpose To investigate the mutations of ID3,TCF3 and MYC genes in Chinese Burkitt lymphoma and discuss their significance.Methods Total DNA was extracted from tumor tissues of 32 patients with Burkitt lymphoma,then the DNA was amplified by polymerase chain reaction (PCR),and the products of PCR were sequenced directly with Sanger sequencing methods.Results The mutation rates of ID3 and TCF3 genes were 35.5％ (11/31) and 18.8％ (6/32) respectively.The mutation rate of MYC was 50％.The mutation rates of MYC exon 1,MYC exon 2 and MYC exon 3 were 3.3％ (1/30),50％ (15/30) and 7.7％ (2/26) respectively.Conclusion Recurrent mutations of the ID3,TCF3 and MYC genes in Chinese Burkitt lymphoma were identified by Sanger sequencing.For TCF3 gene,a novel mutation c.2202G ＞ C p.L569V was found in three cases.In two cases,a novel mutation of c.1070A ＞G p.G182D was found in MYC gene.关键词
淋巴瘤/Burkitt淋巴瘤/ID3/TCF3/MYC/Sanger测序Key words
lymphoma/Burkitt lymphoma/ID3/TCF3/MYC/Sanger sequencing分类
医药卫生引用本文复制引用
魏洁,王哲,范林妮,李侠,李明阳,刘一雄,赵丹珲,闫庆国,郭双平,闫晓初..中国人Burkitt淋巴瘤中ID3、TCF3及MYC基因突变分析[J].临床与实验病理学杂志,2016,32(12):1343-1347,5.基金项目
国家自然科学基金(81570180)、肿瘤生物学国家重点实验室开放基金(CBSKL201504) （81570180）
