临床与实验病理学杂志2016，Vol.32Issue(12)：1361-1364,4.DOI:10.13315/j.cnki.cjcep.2016.12.010
透明细胞肾细胞癌组织中DNMT3B异构体表达及其整体甲基化的分析
Expression of DNA methyltransferase 3B isoforms and global methylation level in clear cell renal cell carcinoma
摘要
Abstract
Purpose The aim of this study was to detect the expressions of DNA methyhransferase 3B isoforms and global methylation level in clear cell renal cell carcinoma (ccRCC) and to discuss the relationship between the changes of them and the ccRCC.Methods Fifteen pairs of tissue samples,which included ccRCC tissue and adjacent matched normal tissue from each patient,were used in this study.Real-time PCR was used to measure the mRNA expression levels of DNMT1,DNMT3A,total DNMT3B,and six kinds of DNMT3B variants.Western blot was used to detect the protein level of DNMT3B4.The repetitive sequences DNA methylation level of Alu and long interspersed nuclear elements (LINE-1) were measured by combine bisulfite restriction analysis (COBRA).Results Both mRNA and protein levels of DNMT3B4 were increased in ccRCC tissue compared with control tissue.Additionally,Alu elements and LINE-1 were hypomethylated in renal cell carcinoma tissue.Conclusion Overexpression of DNMT3B4 may play an important role in human kidney tumorigenesis through the decrease of global methylation.关键词
肾肿瘤/透明细胞肾细胞癌/DNA甲基转移酶3B/整体甲基化Key words
renal neoplasms/clear cell renal cell carcinoma/DNA methyltransferase 3B/global methylation分类
医药卫生引用本文复制引用
高永胜,杨洁,尹树慧,崔钧贺,贾晓娥,谢伟,闫少春,邵国..透明细胞肾细胞癌组织中DNMT3B异构体表达及其整体甲基化的分析[J].临床与实验病理学杂志,2016,32(12):1361-1364,4.基金项目
国家自然科学基金(81160244、81360316、81460283、81550038、81660307)、内蒙古自然科学基金(2014MS0810)、包头医学院博士基金(BSJJ201623、BSJJ201617) （81160244、81360316、81460283、81550038、81660307）
