临床与实验病理学杂志2017，Vol.33Issue(1)：8-11,4.DOI:10.13315/j.cnki.cjcep.2017.01.003
子宫内膜癌中PKD1的表达及其临床意义
Expression and clinical significance of PKD1 in human endometrial carcinoma
摘要
Abstract
Purpose To investigate the expression of protein kinase D1 (PKD1) in endometrial carcinoma and normal endometrium,and to investigate its relationship with the clinicopathological features of endometrial carcinoma.Methods Immunohistochemical SP and qRT-PCR was used to detect the mRNA and protein expression of PKD1 in 92 cases of endometrial cancer and 48 cases of normal endometrium,and to analyze the relationship of expression of PKD1 with the tumor differentiation and clinical stage of endometrial carcinoma tissue.Western blot method was used to detect the expression level of PKD1 protein in normal endometrial cell line and endometrial carcinorna cell lines with different degree of differentiation.Results mRNA and protein expression of PKD1 in endometrial carcinoma tissues was significantly higher than those in normal endometrial tissues (P ＜ 0.01),which showing a correlation to the degree of tissue differentiation and clinical pathologic staging.While,the expression level of PKD1 protein in endometrial cancer cell lines was also much higher than that in normal endometrial cells (P ＜ 0.01),and the lower differentiation,the higher level of PKD1 protein expression.Conclusion PKD1 is highly expressed in endometrial cancer patients.The level of PKD1 expression may be an important reference for predicting the malignant degree of endometrial cancer.关键词
子宫肿瘤/子宫内膜癌/蛋白激酶D1/表达/病理诊断Key words
endometrial neoplasm/endometrial carcinoma/protein kinase D1/expression/pathologic diagnosis分类
医药卫生引用本文复制引用
赵彩琴,钮红丽..子宫内膜癌中PKD1的表达及其临床意义[J].临床与实验病理学杂志,2017,33(1):8-11,4.基金项目
国家自然科学基金(81473438)、河南省科技攻关课题(122102330049) （81473438）
