临床与实验病理学杂志2017，Vol.33Issue(1)：17-21,5.DOI:10.13315/j.cnki.cjcep.2017.01.005
应用时间分辨法检测石蜡包埋子宫颈组织p16INK4a蛋白
Detection p16INK4a protein in cervical tissues with time-resolved fluoroimmunoassay
摘要
Abstract
Purpose To establish a new method for detecting p16INK4a in cervical tissues with time-resolved fluoroimmunoassay (TRFIA).Methods 126 cases of paraffin imbedding tissues of cervix were selected for immunohistochemistry (IHC) of EnVision two-step and TRFIA.Results There were 20 cases of no intraepithelial lesion or malignancy,24 cases of low-grade squamous intraepithelial lesion (LSIL),53 cases of high-grade squamous intraepithelial lesion (HSIL) and 29 cases of squamous cell carcinoma (SCC).In the groups of no intraepithelial lesion or malignancy,LSIL,HSIL and SCC,p161NK4a positive was seen in 1,19,53 and 28,respectively.TRFIA test results displayed p16INK4a positive in 3,17,50 and 27 cases,respectively.Positive of p16 using by TRFIA in no intraepithelial lesion or malignancy,LSIL,above HSIL was 15.00％,70.83％ and 93.90％,respectively (P ＜ 0.01).Conclusion TRFIA is suitable for detecting of p16INK4a protein and demand low detection equipment,p16INK4a expression detected by TRFIA may helpful for large scale detection in various clinical institution.关键词
子宫颈组织/p16INK4a/免疫组织化学/时间荧光分辨法Key words
cervical tissues/p16INK4a/immunohistochemistry/time-resolved fluoroimmunoassay分类
医药卫生引用本文复制引用
丁莉,邹先进,范婉婷,郑杰,陈琼..应用时间分辨法检测石蜡包埋子宫颈组织p16INK4a蛋白[J].临床与实验病理学杂志,2017,33(1):17-21,5.基金项目
湖北省自然科学基金(2013CFB478) （2013CFB478）
