林业科学2017，Vol.53Issue(2)：138-148,11.DOI:10.11707/j.1001-7488.20170216
外源乙烯利与1-MCP处理对桑椹中乙烯和花青素相关代谢基因表达的影响
Effects of Exogenous Ethylene and 1-MCP Treatments on the Expression of Genes Involved in Ethylene and Anthocyanin in Mulberry Fruit
摘要
Abstract
[Objective] Ethylene,a kind of phytohormone,plays a critical role in fruit maturation and senescence.This study aims to explore the effect of ethylene on expression of the genes involved in ethylene of mulberry fruit,in order to provide a basis for exploring the economic value of mulberry using the methods of molecular biology.The total sugar content and anthocyanin content were detected and transcriptional expression of ethylene and anthocyanin related genes were analyzed in the variety 'Jialing 40' (Morus alba 'Jialing 40') fruits.[Method] The mulberry fruits were sprayed with ethephon(100 mg·L-1) 21days (21DAF) and 26 days (26DAF) after full-bloom,respectively and 1-MCP(0.5 μL·L-1) was applied after harvest.Anthocyanin content and total sugar content was measured,total RNA of mulberry were extracted and reverse transcribed to synthesize cDNA.The relative transcriptional expression of ethylene biosynthesis related genes MaACO2 and MaACS3,and signal transduction related genes like MaETR1,MaETR2,MaCTR1,MaEIN2,MaEIL2,and anthocyanin related genes like MaDFR and MaANS were analyzed by using qRT-PCR.[Result] Compared with the control,the anthocyanin and total sugar content were significantly increased by ethephon but decreased by 1-MCP,and the expression level of anthocyanin related genes were up-regulated by ethephon at 21DAF and 26DAF.At 21DAF,the expression level of ethylene related genes were up-regulated by ethephon,but the transcripts of MaACO2,MaACS3 and MaEIL2 were decreased by 1-MCP;MaETR1,MaCTR1,MaEIN2 were up-regulated significantly but the transcripts of MaETR2 displayed a non-significant change by 1-MCP.At 26DAF,the expression level of ethylene related genes were down-regulated by 1-MCP and changed differently by ethephon.After 32 hours,MaACO2,MaETR1,MaETR2,MaEIN2 and MaEIL2 were up-regulated while MaACS3 and MaCTR1 were down-regulated by ethephon compared with the control.[Conclusion] Ethephon could up-regulated the transcripts of ethylene biosynthesis and anthocyanin biosynthesis related genes and influence the ethylene signal transduction related genes,but 1-MCP inhibited the ethylene signal transduction related genes and blocked ethylene signal transduction.Accumulation of anthocyanin and total sugar content were accelerated by ethephon,but inhibited by 1-MCP.关键词
桑/乙烯利/1-MCP/花青素/基因表达/信号转导Key words
Morus alba/ethephon/1-MCP/anthocyanin/gene expression/signal transductions分类
农业科技引用本文复制引用
余建,赵爱春,刘长英,梁燕梅,朱攀攀,蔡雨翔,王茜龄,李镇刚,余茂德..外源乙烯利与1-MCP处理对桑椹中乙烯和花青素相关代谢基因表达的影响[J].林业科学,2017,53(2):138-148,11.基金项目
国家公益性行业(农业)科研专项(201403064) （农业）
国家自然科学基金项目(31360190) （31360190）
国家现代农业产业技术体系建设专项(CARS-22) （CARS-22）
重庆市研究生科研创新项目(CYS2015070). （CYS2015070）
