南京林业大学学报（自然科学版）2017，Vol.41Issue(2)：55-62,8.DOI:10.3969/j.issn.1000-2006.2017.02.009
簸箕柳SPL基因家族分析
Analyzing the SPL gene family in Salix suchowensis
摘要
Abstract
[Objective]This study focused mainly on the differential retention and expansion of the SPL gene family in different species and provided a basis for future study of floral development in angiosperms.[Method]The SPL gene fam?ilies of Arabidopsis thaliana, Populus trichocarpa, Salix suchowensis, Vitis vinifera, Carica papaya and Oryza sativa were identified using homology comparison against the SPL domain. BLASTN was used to identify different types of homologous gene pairs. We used in?house Perl scripts combined with KaKs Calculator to calculate the non?synonymous mutation ( Ka ) and synonymous mutation( Ks ) values of each SPL gene pair. Differential retention and expansion of the SPL gene families of these six angiosperms were determined by synteny analysis. [Result] We detected 120 SPL genes in the ex?amined plants. By analyzing the paralogous and orthologous SPL genes among the six species, we found that the species of Salicaceae have a total of 24 paralogous SPLs and 50 orthologous SPLs, which are the most abundant among the six species;papaya had no paralogous SPL. Among these six species, woody plants had the most abundant orthologous SPLs compared with herbaceous plants, and dicotyledons were found to have more orthologous SPLs than monocotyledons. It is noteworthy that all the Ka/Ks values for the paralogous and orthologous SPL gene pairs were less than 1.0. In transcrip? tome analysis, we detected an SPL gene ( willow GLEAN 10025160) that showed significant different expression in flowering tillers compared to vegetative tillers (P≤ 0.01). [Conclusion]On the basis of bioinformatic analysis, we found that all the Ka/Ks values for the paralogous and orthologous SPL gene pairs were less than 1.0, indicating that the SPL genes in the six examined species have been undergoing purifying selection during the course of evolution, and that their functions should be relatively conserved. We also found that despite whole?genome duplication events, these angio?sperms have undergone large?scale gene loss and gene expansion. Based on the results of sequence collinearity, we ex?plored the intraspecific collinearity of SPL genes and tracked their genome duplication events during the evolutionary his?tory of the six plants. This study provides useful information and clues for further studies of the function of SPL genes in the regulation of plant flowering.关键词
簸箕柳/花发育/SPL基因家族/基因复制Key words
Salix suchowensis flower development/SPL gene family/gene duplication分类
农业科技引用本文复制引用
冯凯,侯静,戴晓港,李淑娴..簸箕柳SPL基因家族分析[J].南京林业大学学报（自然科学版）,2017,41(2):55-62,8.基金项目
国家林业公益性行业科研重大项目(201304102) （201304102）
国家自然科学基金项目(31500533) （31500533）
江苏高校优势学科建设工程资助项目( PAPD) （ PAPD）
