南京林业大学学报（自然科学版）2017，Vol.41Issue(2)：73-80,8.DOI:10.3969/j.issn.1000-2006.2017.02.011
土壤置换法研究土壤基质对微生物碳氮磷的影响
Effects of substrates on soil microbial carbon, nitrogen and phosphorus based on soil replacement method
摘要
Abstract
[Objective]Examine the effects of soil substrates on microbial biomass carbon ( nCmic ) nitrogen ( nNmic ) , and phosphorus ( nPmic ) by comparing their values in the early stage of vegetation succession. [Method]By using the soil dis?placement experiment performed included three treatments. Treatment A involved replacing the original soil of experimen?tal plots ( 0-30 cm depth) with the A-horizon soil of an adjacent temperate broadleaved stand, whereas Treatment B in?volved replacing the soil with B-horizon soil, and treatment C involved replacing the soil with C-horizon soil. The three treatments simulated the secondary succession of clear-cut forests or bare soil without a seed bank or primary succession, respectively, and four replicates were used for each treatment. This study compared the difference of the nCmic , nNmic , nPmic , and their stoichiometry in different soil substrate. [Result]① In 2004, the nCmic , nNmic and nPmic of treatment A were significantly greater than those of treatments B or C ( P < 0.05) , and the nCmic/nNmic ratio of treatment A was lower than those of treatments B or C, whereas the nCmic/nPmic and nNmic/nPmic ratios of treatment A were greater than those of treatments B or C. The nCmic/nC soil( soil carbon) , nNmic/nN soil( soil nitrogen) and nPmic/nP soil( soil phosphor?us) values of treatment A were significantly greater than those of treatments B or C ( P < 0. 05 ) , except for the nNmic/nN soil value of treatment C. ② In 2014, the nCmic , nNmic and nPmic of treatment A were significantly greater than those of treatments B or C ( P< 0.05) , and the nCmic/nNmic ratio of treatments A and B were lower than that of treatment C, whereas the nCmic/nPmic and nNmic/nPmic ratios of treatment A were greater than those of treatments B or C. The nCmic/nC soil , nNmic/nN soil , and nPmic/nP soil values of treatment A were greater than those treatments B or C. ③After the 10-year succession, the nCmic , nNmic and nPmic of the three treatments increased, except for the nPmic of treatment B. The nCmic/nNmic ratio of the three treatments declined, but the nCmic/nPmic and nNmic/ nPmic ratios increased. Among the three treatments, the nCmic/nNmic ratio of treatments B and C decreased significantly ( P < 0.05) , whereas the nNmic/nPmic ratio of treatments A and C increased significantly (P < 0.05). The nCmic/nCsoil, nNmic/nNsoil and nPmic/nPsoil values of three treatments also increased with the succession. [Condusion] The physical and chemical properties of the different sub?strates were the main determinants of the spatial patterns of nCmic , nNmic , nPmic , and their stoichiometry.关键词
土壤基质/演替类型/土壤微生物/化学计量Key words
soil substrate/succession type/soil microbe/stoichiometry分类
农业科技引用本文复制引用
赵娟,王兴昌..土壤置换法研究土壤基质对微生物碳氮磷的影响[J].南京林业大学学报（自然科学版）,2017,41(2):73-80,8.基金项目
"十二五"国家科技支撑计划(2011BAD-37B01) （2011BAD-37B01）
教育部长江学者和创新团队发展计划(IRT-15R09) （IRT-15R09）
