南京林业大学学报（自然科学版）2017，Vol.41Issue(2)：117-121,5.DOI:10.3969/j.issn.1000-2006.2017.02.017
串钱柳挥发油化学成分及其抗细菌活性
Chemical composition and antibacterial activity of volatile oils from the leaves and fruits of Callistemon viminalis
摘要
Abstract
[Objective]Elucidating the chemical composition and antibacterial activity of volatile oils from the leaves and fruits of Callistemon viminalis could provide a theoretical basis for the comprehensive development and utilization of C. viminalis. [Method]Hydro?distillation was used to extract volatile oils from leaves and fruits and their chemical composi?tions were analyzed by gas chromatography?mass spectrometry ( GC?MS) . Furthermore, the filter paper diffusion method was used to detect the inhibitory activity of these oils against seven bacterial species. [Result]The yields of volatile oils from the leaves and fruits of C. viminalis were 0.87% and 0.16% ( based on fresh weight) , respectively. GC?MS analysis identified 14 components in the volatile oil of leaves and 17 components in that of fruits, which accounted for 93. 34%and 90.29%, respectively, of the total volatile oil contents of the two tested samples. Eucalyptol (52.89%), (1R)-(+)-α-pinene (17.28%) and (-)-α-terpineol (10.70%) were the major compounds in the volatile oil of leaves, whereas eucalyptol (38.53%), (1R)-(+)-α-pinene (29.90%) andα-bornene (8.02%) were the major compounds in that of fruits. The fruit volatile oil showed strongest inhibitory activity against Agrobacterium tumefaciens, with an inhi?bition zone diameter of (25.2±2.8) mm. In contrast, the volatile oil from leaves showed strongest inhibitory activity a?gainst Ralstonia solanacearum, with an inhibition zone diameter of (22.3±1.5) mm. [Conclusion]The percentage com? position of volatile oil in the leaves of C. viminalis was higher than that in the fruits. Eucalyptol and ( 1R)-(+)-α-pi?nene were the main volatile oil components in both leaves and fruits. The volatile oil extracted from fruits showed better antibacterial activity against bacteria than the volatile oil extracted from leaves.关键词
串钱柳/挥发油/化学成分/抗细菌活性Key words
Callistemon viminalis/volatile oil/chemical constituent/antibacterial activity分类
农业科技引用本文复制引用
单体江,冯皓,祝一鸣,吴春银,郑明轩,崔紫宁,王军..串钱柳挥发油化学成分及其抗细菌活性[J].南京林业大学学报（自然科学版）,2017,41(2):117-121,5.基金项目
广东省林业科技创新项目(2015KJCH043) （2015KJCH043）
广州市珠江科技新星专项项目(201506010029) （201506010029）
广东省普通高校青年创新人才项目(2014KQNCX034) （2014KQNCX034）
