南京师大学报（自然科学版）2016，Vol.39Issue(4)：65-70,6.DOI:10.3969/j.issn.1001-4616.2016.04.012
不同提取方法对姬菇多糖抗氧化活性的影响
The Effect of Antioxidant Activity on the Polysaccharides from Pleurotus cornucopiae with Different Extraction Ways
摘要
Abstract
Seven different extaction methods of the polysaccharides from pleurotus cornucopiae,including traditional hot water extraction,ultrasonic extraction,ultrasonic wave complex hot water extraction,acid extraction,alkali extraction,ultrasonic wave complex acid extraction and ultrasonic wave complex alkali extraction.And antioxidant activity of the polysaccharides in virto was investigated by using reducing power,hydroxyl radical scavenge activity,superoxide radical assay and DPPH· scavenge activity,using Vc as positive control.The results showed that the polysaccharides prepared by acid extraction had the highest extraction yield that reached 6.58％.In general,the polysaccharides by ultrasonic wave complex hot water extraction reached the highest reducing power(2.18 at 10 mg·mL-1),hydroxyl radical scavenging ability was(95.56％ at 10 mg·mL-1),Superoxide anion radical scavenging activity was(62.35％ at 1 mg·mL-1),DPPH· scavenging ability was (80.32％ at 2 mg· mL-1).The antioxidant activity of the polysaccharides prepared by ultrasonic wave complex alkali extraction was also strong,but the polysaccharides prepared by ultrasonic wave complex acid extraction was the worst.It is concluded that ultrasonic-assisted hot water extraction is an effective method for extracting polysaccharides from pleurotus cornucopiae,and it's more conducive to study the biological activity of polysaccharides.关键词
姬菇多糖/提取率/抗氧化活性/自由基Key words
polysaccharides from Pleurotus cornucopiae/extraction yield/antioxidant activity/free radicals分类
轻工纺织引用本文复制引用
王美菊,陆文娟,喻晨,陶明煊..不同提取方法对姬菇多糖抗氧化活性的影响[J].南京师大学报（自然科学版）,2016,39(4):65-70,6.基金项目
江苏省普通高校研究生科研创新计划项目(KYLX16-1296) （KYLX16-1296）
