南京医科大学学报（自然科学版）2017，Vol.37Issue(2)：189-193,5.DOI:10.7655/NYDXBNS20170211
长非编码RNA BANCR逆转非小细胞肺癌细胞株顺铂耐药的研究
BRAF activated non-coding RNA(BANCR)reverse human non-small cell lung cancer A549/DDP cell line to cisplatin resistance
摘要
Abstract
Objective:To explore the effect of BANCR in reversing cisplatin(DDP) resistance of human non-small cell lung cancer (NSCLC)A549/DDP cell line and its related mechanisms.Methods:The real-time fluorescent quantitative PCR(qRT-PCR) was used to detect BANCR expression differences in DDP-resistant A549/DDP cells and its parent cell line A549.0verexpress BANCR in A549/DDP cells and use MTT assay,colony formation assay and flow-cytometric analysis to detect A549/DDP cells to DDP drug sensitivity,cell proliferation ability and apoptosis on treatment with DDP.The protein expression of p53 in A549/DDP cells were detected by western blotting.Results:The BANCR expression level in A549/DDP cell line was significantly lower than that in A549 cell line (P ＜ 0.05).Ectopic BANCR expression in A549/DDP cells could produce the following effects:compared to the control group,the half inhibition concentration (IC50) of DDP in A549/DDP/BANCR cells lower(P ＜ 0.05),proliferation of A549/DDP/BANCR cells diminished (P ＜ 0.05),apoptosis on treatment with DDP increased (P ＜ 0.05).Furthermore,the protein expressions p53 was upregulated in BANCR transfected group than those in empty vector.Conclusion:BANCR reverses the resistance of A549/DDP cells to DDP through induction of apoptosis,diminished cell proliferation,up-regulating the expressions of p53 protein.关键词
非小细胞肺癌/BANCR/顺铂耐药性Key words
NSCLC/BANCR/DDP-resistance分类
医药卫生引用本文复制引用
姜黎黎,魏晨晨,陈沁楠,陈臻瑶,陈昕,王朝霞..长非编码RNA BANCR逆转非小细胞肺癌细胞株顺铂耐药的研究[J].南京医科大学学报（自然科学版）,2017,37(2):189-193,5.基金项目
国家自然科学基金(81272601,81472198) （81272601,81472198）
江苏省科技厅临床医学科技专项(BL2014096) （BL2014096）
江苏省医学重点人才基金(RC2011080) （RC2011080）
