南京医科大学学报（自然科学版）2017，Vol.37Issue(2)：194-198,5.DOI:10.7655/NYDXBNS20170212
姜黄素-PLGA纳米粒的制备及对人前列腺癌PC-3细胞的体外研究
Preparation of curcumin-loaded PLGA nanoparticles and its effects on human prostate cancer PC-3 cells in vitro
摘要
Abstract
Objective:To prepare curcumin-loaded poly lactic-co-glycolic acid nanoparticles (Cur-PLGA-NPs),to characterize their physicochemical properties,and to study the in vitro release behavior and antitumor activity on human prostate cancer PC-3 cells in vitro.Methods:The Cur-PLGA-NPs were prepared by emulsification-solvent evaporation method.The transmission electron microscope was used to observe the particle appearance,Zetasizer instrument was used to detect the diameter,and ultracentrifugation was utilized to determine drug-loading rate and entrapment rate.Dynamic dialysis method was used to study the in vitro release behavior of Cur-PLGA-NPs.The antitumor activity on PC-3 cells was determined by CCK-8 method.Cells apoptosis and apoptosis rate were examined by using flow cytometry.Ultrastructural changes of cells were observed under the electronic microscopy,and compared to free Cur groups.Results:The optimal NPs were round with the nanometric size of (165.36 ± 24.21)nm,high entrapment rate of (83.05 ± 1.07)％ and drug-loading rate of (10.87 ± 0.58)％.In vitro release test showed that release rate within 24 h of CurPLGA-NPs was 44.02％,and accumulating release rate was 84.81％ at day10.After an effection of 5～40 μmol/L Cur and Cur-PLGANPs on PC-3 cells for 24～72 h,the inhibition rates of Cur and Cur-PLGA-NPs were 9.38％～83.62％ and 10.56％～89.53％.CCK-8 experience revealed that there was significant difference between 20 μmol/L group and 40 μmol/L group compared Cur with CurPLGA-NPs(P ＜ 0.05).Flow cytometry indicated that there was a significant difference between experiment group and control group(P ＜ 0.05).Transmission electron microscopy showed that Cur-PLGA-NPs induced morphological change of apoptosis on PC-3 cells.Conclusion:The Cur-PLGA-NPs has good drug sustained-release capacity in vitro,and significantly improves the capacity of Cur to kill and inhibit the proliferation of PC-3 cells in vitro.关键词
姜黄素/纳米粒/聚乳酸-羟基乙酸/前列腺癌/抗肿瘤Key words
curcumin/nanoparticles/PLGA/prostate cancer/antitumor activity分类
医药卫生引用本文复制引用
钟思文,姜锡男,陈方敏,石家齐,李登宝,陈绪龙,唐帅,陈程..姜黄素-PLGA纳米粒的制备及对人前列腺癌PC-3细胞的体外研究[J].南京医科大学学报（自然科学版）,2017,37(2):194-198,5.基金项目
国家自然科学基金(30860284) （30860284）
贵阳市科技攻关(2009筑科农合同字第3-009号) （2009筑科农合同字第3-009号）
贵州省教育厅重点项目(黔教科2007027) （黔教科2007027）
