宁夏大学学报（自然科学版）2017，Vol.38Issue(1)：78-82,5.
mTOR在人脂肪源间充质干细胞成脂中的作用
Effect of mTOR on Adipogenesis of Human Mesenchymal Stem Cells
摘要
Abstract
By studying the influence of mTOR on hADSCs adipogenesis,the article provides reference for prevention and treatment of adipocyte prosoplasia related diseases.Specific to the human abdominal adipose tissues extracted by liposuction,Ⅰ collagenase was used to digest and seperate hADSCs,triple inducement method made the differentiation of adipose adipocyte,and oil red O staining identify.The result shows that,the isolated cells are fusiform cell with parallel arrangement and comparatively even in growth form.7 days after inducing via triple induction method shows visible lipid drops,and oil red O redcolored.The distribution of mTOR turns from intranuclear to cytoplasm during the process of atomization.The processing of cells by sirolimus made the accumulation of lipid droplet in cells reduce significantly and the express of PP PPARγ'decrease.The restraining of sirolimus on mTOR express can decrease the adipogenesis ability of hADSCs.关键词
脂肪分化/人脂肪源间充质干细胞/mTORKey words
adipose differentiation/mesenchymal stem cell in human adipose/mTOR分类
医药卫生引用本文复制引用
张永久,李德华..mTOR在人脂肪源间充质干细胞成脂中的作用[J].宁夏大学学报（自然科学版）,2017,38(1):78-82,5.基金项目
辽宁省自然科学基金资助项目(20102138) （20102138）
