农业环境科学学报2017，Vol.36Issue(2)：279-285,7.DOI:10.11654/jaes.2016-0991
生物修复对石油污染土壤微生物活性的影响
Impacts of bioremediation on microbial activities in petroleum contaminated soil
摘要
Abstract
Biodegradation of petroleum hydrocarbons in oil-polluted soil is carried out by various microorganisms.But little information is available for the relationships between hydrocarbon degradation and soil enzyme and microbial metabolic activities.In this study,petroleum contaminated soils which were collected from the North of Shaanxi Province of China were treated by using the bioremediation method.The changes of different fractional of petroleum hydrocarbons were determined using the gravimetry method.Four enzyme activities (polyphenoloxidase,catalase,dehydrogenase,and lipase) were determined by the method of spectrophotometry and titration.The hydrocarbon degrading activities of soil microorganisms were determined by Biolog(MT2) Micro Plates assay.The correlations between the different fractional of hydrocarbons and biotic factors were analyzed by using SPSS 19.0 software.Results showed that bioremediation was an effective method for removal of petroleum hydrocarbons,which promoted respectively 46.8％ and 39.9％ degradation of alkanes and polycyclic aromatic hydrocarbons (PAHs) after 11 weeks of remediation.The catalase and lipase activities,as well as the hydrocarbon utilization abilities of soil microorganisms were enhanced during bioremediation.However,polyphenoloxidase and dehydrogenase activites increased firstly and then decreased.The correlation analysis results indicated alkane degradation was related to alkane metabolic activities,as well as dehydrogenase and catalase activities.PAHs degradation was related to dehydrogenase and catalase activities.The results suggest that bioremediation enhanced the correlation relationships between the different fractional hydrocarbon degradation and soil microbial activities.关键词
石油污染土壤/微生物修复/土壤酶活/烃代谢活性/相关性分析Key words
petroleum contaminated soil/bioremediation/soil enzyme activities/hydrocarbon utilization/correlation analysis分类
资源环境引用本文复制引用
陈凯丽,吴蔓莉,叶茜琼,李炜,袁婧..生物修复对石油污染土壤微生物活性的影响[J].农业环境科学学报,2017,36(2):279-285,7.基金项目
国家自然科学基金项目(21577109) （21577109）
陕西省自然科学基础研究基金面上项目(2015JM5163) （2015JM5163）
陕西省教育厅重点实验室科学研究计划项目(13JS048)The National Natural Science Foundation of China(21577109) （13JS048）
The Natural Science Foundation of Shaanxi Province,China(2015JM5163) （2015JM5163）
The Scientific Research Project of Shaanxi Province Department of Education Key Laboratory(13JS048) （13JS048）
