四川大学学报（自然科学版）2017，Vol.54Issue(1)：161-166,6.DOI:103969/j.issn.0490-6756.2017.01.027
猪抑胃肽/葡萄糖依赖性促胰岛素释放肽(GIP)基因的克隆与组织表达分析
Cloning and tissue expression of gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) in pigs
摘要
Abstract
The gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) shows the inhibitory effect on gastric acid secretion and incretin action.The aim of this study is to analyze the cDNA sequence of the pig GIP (pGIP) gene and further examine its the tissue expression.Result showed that pGIP cDNA is 435 bp in length and encodes a 144-amino acid GIP precursor which contains a signal peptide and mature GIP peptide.RT-PCR assay showed that pGIP mRNA is highly expressed in kidney and gastrointestinal tract.关键词
猪/GIP/分子克隆/结构分析/组织表达Key words
Pig/GIP/Molecular cloning/Structure analysis/Tissue expression分类
生物科学引用本文复制引用
程绍臣,张剑南,钟宇,李娟,王亚军..猪抑胃肽/葡萄糖依赖性促胰岛素释放肽(GIP)基因的克隆与组织表达分析[J].四川大学学报（自然科学版）,2017,54(1):161-166,6.基金项目
国家转基因生物新品种培育重大专项(2009zx08009-145B) （2009zx08009-145B）
