药学研究2017，Vol.36Issue(3)：132-134,3.DOI:10.13506/j.cnki.jpr.2017.03.002
一种中药提取物的制备及对东莨菪碱模型小鼠学习记忆能力的影响
Preparation of self-made compound extract of Chinese medicine and its effect on learning-memory ability in model mice induced by scopolamine
摘要
Abstract
Objective To optimize the extraction technology for the self-made of Chinese medicine and investigate the effect of the extraction on learning-memory ability in model mice induced by Scopolamine.Methods The dry extract yield was chosen as index.The four factors of the extraction solvent and time,the solvent ratio,and soaking time were investigated by L9(34) orthogonal test.Kunming mice were randomly divided into normal control group,scopolamine model group,positive control drug,low,medium and high doses of the self-made of Chinese medicine(0.5,1.0 and 2.0 g·kg-1).The drugs were intragastrically administered to mice for four weeks.On day 28,after intraperitoneal injection of scopolamine,mice were subjected to Morris water maze tests.Results The optimal technology was added to 12 times the amount of 50% ethanol,soaking 90 min,refluxing and extracted three times,each extraction for 2 hours.In animal experiments a significant decrease of swimming time in Morris water maze test was observed in the self-made of Chinese medicine groups(P<0.05).Conclusion The optimal technology for the self-made compound extract of Chinese medicine was reasonable,the extraction from which could effectively improve learning-memory ability in model mice induced by scopolamine.关键词
自制中药/出膏率/正交试验/提取工艺/学习记忆/东莨菪碱Key words
Self-made compound extract of Chinese medicine/Dry extract yield/Orthogonal test/Extraction technology/Learning and memory ability/Scopolamine分类
医药卫生引用本文复制引用
王芳,梁大连,郭琪,程艳玲,于亮,黄艳妮..一种中药提取物的制备及对东莨菪碱模型小鼠学习记忆能力的影响[J].药学研究,2017,36(3):132-134,3.基金项目
国家重大新药创制项目(No.2010ZX09401302-002-015) （No.2010ZX09401302-002-015）
