药学研究2017，Vol.36Issue(3)：135-137,3.DOI:10.13506/j.cnki.jpr.2017.03.003
高效液相色谱法对大川芎片中5种成分的含量测定
Determination of 5 components of Dachuanxiong Tablets by HPLC method
摘要
Abstract
Objective To establish the fingerprint of Dachuanxiong Tablets by HPLC methods,and determine the 5 kinds of index components contents such as senkyunolide Ⅰ,chlorogenic acid,senkyunolide A,vanillic acid,ferulic acid.Methods Agilent poroshell SB-C18 column(4.6 mm×100 mm,2.7 μm) was adopted,the mobile phase was 0.02% formic acid water(A)-acetonitrile(B) solution with gradient elution,the detection wavelength was 280 nm, the flow rate was 0.8 mL·min-1,the column temperature was 30 ℃,and the recording time was 40 min.The contents of 10 different batches of Dachuanxiong Tablets from same manufacturer were determined.Results This study established the HPLC fingerprint of Dachuanxiong Tablets,the index component of senkyunolide Ⅰ,chlorogenic acid,senkyunolide A,vanillic acid and ferulic acid were separated,the linear range was 3~48 nL(r=0.999 9),0.062 2~0.995 5 μg(r=0.999 8),0.3~4.8 nL(r=0.999 9),0.026 6~0.426 2 μg(r=0.999 9),0.016 9~0.270 7 μg(r=0.999 7),respectively.The recoveries were ranged from 95% to 105%,and the precision,stability and repeatability were good.Conclusion This study used senkyunolide Ⅰ,chlorogenic acid,senkyunolide A,vanillic acid and ferulic acid as the index component for the determination of Dachuanxiong Tablets,the method was accurate and reproducible which can reflect the comprehensive quality of Dachuanxiong Tablets and can lay the foundation for quality control of Dachuanxiong Tablets.关键词
大川芎片/含量测定/高效液相色谱法Key words
Dachuanxiong Tablets/Determination/HPLC method分类
医药卫生引用本文复制引用
马钰,王帅,孟宪生,富力,英锡相..高效液相色谱法对大川芎片中5种成分的含量测定[J].药学研究,2017,36(3):135-137,3.基金项目
辽宁省自然科学基金(No.201602476) （No.201602476）
