山东医药2017，Vol.57Issue(12)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.12.001
T2DM合并冠心病患者与单纯T2DM患者血浆差异表达微小RNA筛选
Screening out miRNAs differentially expressed between T2DM patients with and without coronary heart disease
摘要
Abstract
Objective To screen out the microRNAs (miRNAs) differentially expressed between type 2 diabetes mellitus (T2DM) patients with and without coronary heart disease.Methods Clinical data were obtained from 30 cases of T2DM patients with coronary heart disease and 30 cases of T2DM patients without coronary heart disease, respectively.miRNA identification was performed by microarray for differentially expressed miRNAs and their expression was confirmed by real-time fluorescence quantification RT-PCR.The relationships between the differentially expressed miRNAs and clinical parameters were analyzed.Results According to the screening of microarray, 7 different miRNAs were selected as candidate miRNAs, including miR-765, miR-320c, miR-33b-3p, miR-21-5P, miR-22-3p, miR-20b-5p and miR-92a-3p.The expression of miR-765 and miR-320c was down-regulated in T2DM patients with coronary heart disease as compared with that of T2DM patients without coronary heart disease (both P<0.01).The expression of miR-765 and miR-320c was negatively correlated with HbA1C (β=-0.379, β=-0.192, both P<0.01) and LDL-C (β=-0.224, β=-0.232, both P<0.05).Conclusion miR-765 and miR-320c express differently between T2DM patients with and without coronary heart disease, which may be the potential biomarkers for T2DM patients with coronary heart disease.关键词
2型糖尿病/冠状动脉粥样硬化性心脏病/基因芯片/微小RNAKey words
type 2 diabetes mellitus/coronary atherosclerotic heart disease/microarray/microRNA分类
医药卫生引用本文复制引用
祝茸,孙卫平,闫宇翔,张玲,何燕..T2DM合并冠心病患者与单纯T2DM患者血浆差异表达微小RNA筛选[J].山东医药,2017,57(12):1-4,4.基金项目
国家自然科学基金资助项目(31672375/30971178). （31672375/30971178）
