山东医药2017，Vol.57Issue(12)：8-11,4.DOI:10.3969/j.issn.1002-266X.2017.12.003
人脂肪组织单核细胞趋化蛋白1表达变化及意义
Expression of monocyte chemoattractant protein-1 in human adipose tissues
查娟民 1李华善 2林倩 2丁国宪 2何伟奇3
作者信息
- 1. 苏州大学附属第一医院,江苏苏州215006
- 2. 苏州大学剑桥-苏大基因组资源中心
- 3. 南京医科大学附属第一医院
- 折叠
摘要
Abstract
Objective To investigate the expression of monocyte chemoattractant protein-1 (MCP-1) in human adipose tissues and its significance.Methods Epiploic fatty tissues were collected from patients with cholecystolithiasis for selective surgery.The mRNA expression of tumor necrosis factor-α (TNF-α), MCP-1, and toll-like receptor 4 (TLR4) was measured by real-time fluorescence quantitative RT-PCR.The regression analysis was used to examine the relationship between the expression levels of these factors and body mass index (BMI).Preadipocytes were isolated from epiploic fatty tissues in patients with cholecystolithiasis for selective surgery and were cultured in medium.Cell morphological changes were observed under the microscope.Cells were randomly divided into two groups: the observation group was given a final concentration of 500 g/ml free fatty acid (FFA) for the treatment, and the control group was cultured with primary medium.Twenty-four hours after treatment, the relative mRNA expression of TNF-α, MCP-1, and TLR4 was measured by using real-time fluorescence quantitative RT-PCR.Results The mRNA expression levels of MCP-1, TNF-a, and TLR4 were 1.20±0.07, 0.57±0.07, and 1.22±0.08, respectively.The expression of MCP-1 in adipose tissues was positively correlated with BMI (r2=0.2343, P<0.05);the expression of TNF-α was also positively correlated with BMI (r2=0.428 6, P<0.01).The expression of MCP-1 (P<0.05) and TNF-α (P<0.05) increased after FFA treatment.No significant difference was found in the expression of TLR4 between these two groups (P>0.05).Conclusion The expression of MCP-1 in adipose tissues increases with the increase of BMI, and the MCP-1 expression is increased and inducing obesity may be caused by regulating TLR4 signaling pathway and promoting the release of TNF-α.关键词
脂肪组织/单核细胞趋化蛋白1/肿瘤坏死因子α/胰岛素抵抗/体质量指数Key words
adipose tissues/monocyte chemoattractant protein-1/tumor necrosis factor-α/insulin resistance/body mass index分类
医药卫生引用本文复制引用
查娟民,李华善,林倩,丁国宪,何伟奇..人脂肪组织单核细胞趋化蛋白1表达变化及意义[J].山东医药,2017,57(12):8-11,4.
