山东医药2017，Vol.57Issue(12)：16-19,4.DOI:10.3969/j.issn.1002-266X.2017.12.005
弥漫大B细胞淋巴瘤患者骨髓中淋巴瘤相关巨噬细胞数量变化及临床意义
Levels of lymphoma-associated macrophages in bone marrow of patients with diffuse large B-cell lymphoma and its clinical significance
摘要
Abstract
Objective To investigate the levels of lymphoma-associated macrophages (LAM) in bone marrow of patients with diffuse large B-cell lymphoma (DLBCL) and its clinical significance.Methods Forty DLBCL patients (DLBCL group) and 18 healthy controls (NC group) were enrolled in the study.M2 macrophages (LAM) in the bone marrow and the ratio of M2 to M1 (M2/M1) in the two groups were detected by flow cytometry (FCM).C-C motif chemokine 22 (CCL22) mRNA expression was analyzed using RT-PCR.The relationship between the percentage of M2 macrophages in bone marrow and CCL22mRNA, lactate dehydrogenase (LDH), β2 microglobulin (β2-MG) was analyzed by Pearson correlation analysis.Results The M2 percentages in the DLBCL group and NC group were 11.38%±1.23% and 4.53%±0.83% , respectively (P<0.05).The M2/M1 ratios in the DLBCL group and NC group were 0.75±0.08 and 0.61±0.07, respectively (P<0.05).The CCL22 mRNA expression in the bone marrow mononuclear cells of the DLBCL group and NC group was 4.64±0.73 and 2.12±0.76, respectively (both P<0.05).The M2 percentage and M2/M1 ratio and the CCL22 mRNA expression in the bone marrow mononuclear cells of patients with initial treatment and progression of disease (PD) were higher than those in the effectively treated and stable disease (SD) patients (both P<0.05).The M2 percentage in the IPI score 0-1 group was lower than in the IPI score ≥2 group (P<0.05).The M2 percentage in stage I-III patients was lower than that in the stage Ⅳ patients (P<0.05).The M2 percentage in patients with B symptom was higher than in patients without B symptom (P<0.05).There were positive correlations between the M2 percentage and the CCL22 mRNA expression, LDH level and β2-MG level (r=0.37, 0.49, 0.42, all P<0.05).Conclusion The increased amount of LAM in bone marrow of patients with DLBCL is related to disease progression, curative effect and prognosis, which can reflect the tumor burden and promote the expression of CCL22.关键词
弥漫大B细胞淋巴瘤/淋巴瘤相关巨噬细胞/CC类趋化因子22Key words
diffuse large-B-cell lymphoma/lymphoma-associated macrophages/C-C motif chemokine 22分类
医药卫生引用本文复制引用
王艳,邢莉民,徐文艳,王化泉,苏心,邵宗鸿..弥漫大B细胞淋巴瘤患者骨髓中淋巴瘤相关巨噬细胞数量变化及临床意义[J].山东医药,2017,57(12):16-19,4.基金项目
天津市留学回国人员科研启动基金(津人才办[2015]31号). （津人才办[2015]31号）
