上海农业学报2017，Vol.33Issue(1)：138-143,6.DOI:10.15955/j.issn1000-3924.2017.01.24
猪MⅡ期卵母细胞玻璃化冷冻后的凋亡途径研究
The apoptotic pathways of vitrified porcine oocytes at MⅡ stage
摘要
Abstract
In order to clarify the appopotic pathways of MⅡ-stage porcine oocytes after vitrification,the Caspase 3,Caspase 8,Caspase 9 and Pan-caspase activities of vitrified porcine oocytes in death receptor mediated and mitochondrion mediated apoptotic pathways were determined by in situ fluorescence staining,and the mRNA expressions of key genes in different pathways were also detected by RT-PCR.The results were as follows:After vitrification of the MⅡ-stage oocytes the fluorescence intensity values of Caspase 8,Caspase 9,Caspase 3 and Pan-caspase were respectively 32.03,16.56,16.70 and 8.43,and significantly higher than those of fresh oocytes' control groups(being 4.02,4.83,4.23 and 3.08 respectively).After vitrification the expression levels of TNFα,FasL,CASP8 and CASP3 genes had a certain increasing,those of CASP9,CASP3 and P53 genes presented an upward trend,whereas those of Bcl2,BAX,SOD1 and survivin genes significantly decreased.It was concluded that the apoptosis of MⅡ-stage porcine oocytes after freezing was implicated in both death receptor mediated and mitochondrion mediated apoptotic pathways.关键词
猪/卵母细胞/玻璃化/细胞凋亡Key words
Pig/Oocyte/Vitrification/Apoptosis分类
农业科技引用本文复制引用
吴彩凤,戴建军,钮莹芳,张树山,陈亚宁,张德福..猪MⅡ期卵母细胞玻璃化冷冻后的凋亡途径研究[J].上海农业学报,2017,33(1):138-143,6.基金项目
国家自然科学基金(31372315) （31372315）
国家转基因生物新品种培育科技重大专项(2014ZX08006-005) （2014ZX08006-005）
上海市农业委员会青年人才计划(2014-1-32,2014-1-33) （2014-1-32,2014-1-33）
