世界中医药2017，Vol.12Issue(1)：147-151,5.DOI:10.3969/j.issn.1673-7202.2017.01.037
培元解郁方对慢性应激BALB/c小鼠的抗抑郁作用及TRY-KYN代谢途径的调节
Antidepressant Effects of Peiyuan Jieyu Decoction on Chronic Stress BALB/C Mice and Its Regulatory Mechanism on TRY-KYN Metabolism
摘要
Abstract
Objective:To study the influence of antidepressant effects of Sini Powder,Tiansi Liquid,and Peiyuan Jieyu Decoction on corticosrone and tryptophan 2,3-double enzyme (TDO) and key metabolism enzyme of the tryptophan-kynurenine (TRP-KYN) pathway.Methods:The mice model was established by chronic stress.The immobility time in forced swimming test was acquired and analysed by Noldus EthoVision XT9 system.Tryptophane,kynurenine,kynurenic acid,quinaldinic acid and corticosrone in serum were detected by HPLC-MS/MS technique.The mRNA expression level of TDO in liver was detected by real-time PCR.Results:After five days chronic stress,the immobility time of model group mice was prolonged.The serum corticosterone levels,KYN/TRP and KYN/KA specific value increased and KYN/Q specific value decreased.Tiansi Liquid,Sini Powder and Peiyuan Jieyu Decoction could shorten the immobility time of mice (P ＜0.1).It could also cut the corticosterone levels (P ＜0.05);increased KYN/TRP and KYN/Q specific value (P ＜ 0.05) and reduced the KYN/KA specific value.Conclusion:Sini Powder,Tiansi Liquid and Peiyuan Jieyu Decoction have antidepressant effect.The mechanism may be related to the increase of serum corticosterone induced by stress reaction and the reduce of neurotoxicity during the TRY-KYN metabolim and improvement of nerve protection.关键词
四逆散/天丝饮/培元解郁方/抗抑郁/色氨酸-犬尿氨酸Key words
Sini Powder/Tiansi Liquid/Peiyuan Jieyu Decoction/Anti-depression/TRY-KYN分类
医药卫生引用本文复制引用
李海娜,张静,李丽娜,范盎然,于雪,王萍萍,刘保秀,畅洪昇..培元解郁方对慢性应激BALB/c小鼠的抗抑郁作用及TRY-KYN代谢途径的调节[J].世界中医药,2017,12(1):147-151,5.基金项目
国家自然科学基金项目(编号:81373584) （编号:81373584）
