世界中医药2017，Vol.12Issue(3)：499-503,5.DOI:10.3969/j.issn.1673-7202.2017.03.005
逍遥散对肝郁脾虚证大鼠胃组织基因表达谱的影响
Effects of Xiaoyao Powder on stomach gene expression profile of rats with liver-depression and spleen deficiency
摘要
Abstract
Objective:To systematically investigate the different effects of largemedium and small dose of Xiaoyao Powder on gene expression profile in stomach tissue of rats with the syndrome of liver-depression and spleen-deficiency induced by chronic immobilization stress and to study the anti-stress regulatory mechanism of the three different doses at the whole genome.Methods:The rat whole genome expression chips (Agilent) were used to detect the differences of gene expression in the Stomach of the rat model with the syndrome of liver-depression and spleen-deficiency induced by 21-day chronic immobilization stress(3 hours per day) in normal groupmodel group and Xiaoyao Powder groups (largemedium and small dose,respectively)and any differential gene expression profiles between groups were recognized and selected.Then the differential gene expression profiles were analyzed through gene ontology and signal pathway using bioinformatics.Results:The differential gene expression profiles of model group and Xiaoyao Powder groups (largemedium and small dose) were different.The functions of multiple biological processes and signaling pathways were significantly up-regulated or down-regulated.The significantly-changed biological processes participated by differential gene of the model group and the Xiaoyao Powder groups(Largemedium and small dose) had 597,1216,817,924 function gene groups respectivelyand signal pathways had 27,60,41,59 respectively.Conclusion:The syndrome of liver-depression and spleen-deficiency has the background of differentially expressed genomics in stomach.The largemedium and small dose of Xiaoyao Powder all have certain intervention effects on multiple stomach differentially expressed genesthe signal pathways and the biological processes whose functions are both changed.The result shows the medium dose of Xiaoyao Powder is better than the large and small dose.关键词
逍遥散/肝郁脾虚证/胃组织/基因表达谱Key words
Xiaoyao powder/Syndrome of liver depression and spleen deficiency/Stomach tissue/Gene expression profile分类
医药卫生引用本文复制引用
李晓红,陈家旭,张煜,臧知明,杨力强..逍遥散对肝郁脾虚证大鼠胃组织基因表达谱的影响[J].世界中医药,2017,12(3):499-503,5.基金项目
国家自然科学基金项目(编号:81360526 （）
81560750) （）
广西自然科学基金项目(编号:2013GXNSFAA019129) （编号:2013GXNSFAA019129）
广西特色实验动物病证模型重点实验室建设项目(编号:桂教科研[2014]14) （编号:桂教科研[2014]14）
