世界中医药2017，Vol.12Issue(3)：504-508,512,6.DOI:10.3969/j.issn.1673-7202.2017.03.006
逍遥散对肝郁脾虚证大鼠下丘脑基因表达谱的影响
Effects of Xiaoyao Powder on hypothalamus gene expression profile of rats with liver-stagnation and spleen-deficiency syndrome
摘要
Abstract
Objective:To systematically investigate the effect of Xiaoyao powder on hypothalamus gene expression profile of rats with syndrome of liver-depression and spleen-deficiency induced by chronic immobilization stress and its anti-stress regulatory mechanism at the whole genome.Methods:The rat whole genome expression chips(Agilent) were used to detect gene expression differences in the hypothalamus of the rat model with liver-stagnation and spleen-deficiency syndrome induced by chronic immobilization stress(daily immobilization stress for 3 h for 21 days) in normal groupmodel group and Xiaoyao powder group(largemedium and small doses) and any different gene expression profiles between groups were recognized and selected.The hypothalamus gene expression profile was studied through gene ontology and signal pathway analyses using bioinformatics.Results:The differential gene expression profiles of model group and Xiaoyao Powder groups (largemedium and small dose) were different.The functions of multiple biological processes and signaling pathways were significantly up-regulated or down-regulated.The significantly-changed biological processes participated by differential gene of the model group and the Xiaoyao Powder groups(Largemedium and small dose) had909,712,516,1322 respectivelyand signal pathways had 60,57,41,90 respectively.Conclusion:The liver-stagnation and spleen-deficiency syndrome has the background of differentially expressed genomics in hypothalamus.The largemedium and small doses of Xiaoyao powder all have a certain intervention effect on multiple hypothalamus differentially expressed genessignal pathway and biological process whose functions are both changed.The result shows the large and medium doses are better than the small dose.关键词
逍遥散/肝郁脾虚证/下丘脑/基因表达谱Key words
Xiaoyao Powder/The Live-stagnation and Spleen Deficiency Syndrome/Hypothalamus/Gene Expression Profile分类
医药卫生引用本文复制引用
李晓红,陈家旭,孔鹏云,肖艳芬,杨力强..逍遥散对肝郁脾虚证大鼠下丘脑基因表达谱的影响[J].世界中医药,2017,12(3):504-508,512,6.基金项目
国家自然科学基金项目(编号:81360526 （）
81560750) （）
广西自然科学基金项目(编号:2013GXNSFAA019129) （编号:2013GXNSFAA019129）
广西特色实验动物病证模型重点实验室建设项目(编号:桂教科研[2014]14) （编号:桂教科研[2014]14）
