世界中医药2017，Vol.12Issue(3)：618-622,5.DOI:10.3969/j.issn.1673-7202.2017.03.036
丹参酮ⅡA磺酸钠注射液对兔心肌缺血预处理后血管内皮损伤的保护作用
Protective effect of Danshen A Sodium Sulfonate Injection on Vascular Endothelial Injury after Ischemic Preconditioning in Rabbits with Myocardial Ischemia Preconditioning
摘要
Abstract
Objective:To observe the protective effect of Danshen A sodium sulfonate injection on myocardial ischemia and reperfusion injury in rabbit,investigate the effect of Danshen A sodium sulfonate injection on myocardial enzyme spectrum and explore its potential mechanism to heart function.Methods:Twenty-four rabbits were randomly divided into ischemia/reperfusion group,ischemic preconditioning processing group A,and ischemic preconditioning group B.Ischemic preconditioning processing group A was injected via ear vein with Danshen A sodium sulfonate injection at 2.5 mL/kg/d,while ischemic preconditioning treatment group B at 5 mL/kg/d and ischemia/reperfusion group were injected with 1 mL physiological saline.After a week preprocessing,rabbits in all groups were ligated at coronary artery and was re-perfused for 30 minutes,30 minutes after ligation.The rest 8 rabbits were fed up as control group.Vascular endothelial growth factor (VEGF) and VEGF mRN expression before and after processing were measured by double antibody sandwich EUS,and serum total superoxide dismutase (T-SOD),lactate dehydrogenase (LDH),creatine kinase isoenzyme (CK-MB) and myocardial malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay (ELIS).The protective effect of Danshen A sodium sulfonate injection on vascular endothelial injury and its effect on myocardial enzyme spectrum in rabbits after myocardial ischemia preconditioning were evaluated.Results:Differences of the four groups in ischemic preconditioning showed no significance (P>0.05),VEGF and mRN expression in both group A and B after preprocessing were significantly enhanced,and T-SOD,LDH and CK-MB of were obviously improved and the release of MD declined in the two groups with statistically significant difference compared with the ischemia/reperfusion group (P<0.05).There was no significant difference between the ischemic preconditioning group A and group B (P>0.05).Conclusion:Danshen A sodium sulfonate injection on myocardial ischemia and reperfusion injury in rabbits may significantly increase VEGF and enhance LDH activity,accelerate myocardial ischemia vascular endothelial growth,inhibit lipid peroxidation inhibition and reduce myocardial vascular endothelial inflammatory response,thereby improving impaired endothelial function due to ischemia/reperfusion injury.It has clear protective effects on rabbit experimental myocardial ischemia/reperfusion injury.关键词
丹参酮ⅡA磺酸钠注射液/心肌缺血/再灌注损伤预处理/血管内皮生长因子/心肌酶谱Key words
Danshen A sodium sulfonate injection/Myocardial ischemia/reperfusion injury preconditioning/Vascular endothelial growth factor/Myocardial enzyme spectrum分类
医药卫生引用本文复制引用
刘家军,吴松,李龙珠,敖金波..丹参酮ⅡA磺酸钠注射液对兔心肌缺血预处理后血管内皮损伤的保护作用[J].世界中医药,2017,12(3):618-622,5.基金项目
湖北省教育厅教育科学"十二五"规划课题(编号:2014B095) （编号:2014B095）
