森林工程2017,Vol.33Issue(2):17-21,5.
红松不同种源的遗传多样性分析
Study on the Genetic Diversity of Pinus Koraiensisin Different Provenances
摘要
Abstract
Pinus koraiensis is the main tree species in Xiaoxing'anling forest,and of important ecological status in the overall ecological system of Xiaoxing'anling area.In order to ensure the genetic richness of Pinus koraiensis in Xiaoxing'anling forest region,and provide high quality Pinus koraiensis afforestation seedlings for Xiaoxing`anling forest area,10 primers were screened and 10 different Korean pine natural provenances were analyzed by ISSR in this study.The results showed that the points were detected by 10 ISSR primers were between 2~13loci,DNA fragment length of each point was between 150~2000 bp.79 loci were detected,and 9.9 loci was detected by ISSR primers on the average.Polymorphic bands for the 65 polymorphic loci was 42.63%.There was a significant difference in the molecular level of the various sources,and this difference was positively correlated to the geographical distribution.The genetic richness of Pinus koraiensis community was higher in Xiaoxing'anling forest area,which effectively ensured the quality of Pinus koraiensis community in Xiaoxing'anling.关键词
红松/遗传丰富度/ISSRKey words
korean pine/genetic richness/ISSR分类
农业科技引用本文复制引用
张巍,王清君,郭兴..红松不同种源的遗传多样性分析[J].森林工程,2017,33(2):17-21,5.基金项目
黑龙江省森林工业总局科技攻关项目(sgzjy2010005) (sgzjy2010005)