森林工程2017，Vol.33Issue(2)：33-40,44,9.
紫叶白桦组培苗叶片花青素含量影响因素分析
Analysis of Factors Influencing on Anthocyanin Content inLeaves of Tissue Culture Plantlet of Royal Frost Betula
摘要
Abstract
The Content of anthocyanin of plantlets leaves and the proportion of anthocyanin,chlorophyll,flavonoid,carotenoid and total phenol was measured in vitro rapid propagation by taking royal Frost Betula as the experimental material.The effects of environmental factors on the color change of leaves and the content of anthocyanins were analyzed.The results showed that WPM proliferation medium was useful to the accumulation of anthocyanins.The synthesis of anthocyanin was inhibited with the increase of hormone concentration.There were no differences in hormone types during the inhibitory effect.Lacking sucrose in the medium reduced the accumulation of anthocyanins.The lower PH value and temperature had positive effects on the accumulation of anthocyanins.Prolonged illumination time can facilitate the accumulation of anthocyanins as well.Strong illumination can improve the contents of anthocyanin in microscopes of tissue culture and leaves of regeneration plant while the content and proportion of chlorophyll decreased.Contents of flavonoid and total phenol decreased with the anthocyanin decreasing.The proportion of chlorophyll and carotenoid increased with the decrease of anthocyanin.The results of this research provided the theory guidance and reference for cultivating quality ornamental nursery stock of Royal Frost Betula.关键词
紫叶白桦/离体快繁/花青素/叶绿素Key words
Royal Frost Betula/in vitro rapid propagation/anthocyanin/chlorophyll分类
农业科技引用本文复制引用
杨玲,刘虹男,张冬严,沈海龙..紫叶白桦组培苗叶片花青素含量影响因素分析[J].森林工程,2017,33(2):33-40,44,9.基金项目
黑龙江省博士后科研启动金资助项目(LBH-Q14003) （LBH-Q14003）
