森林工程2017，Vol.33Issue(2)：41-44,4.
落叶松-杨栅锈菌效应因子Mlp-AvrM基因克隆
The Effect Factor Mlp-AvrM GeneCloning ofMelampsora larici-populina
摘要
Abstract
To investigate the interaction mechanism of Melampsora larici-populina and poplar and the role that ufnencing factors play in the process of resistance,this research cloned Melampsora larici-populina effect factor:Mlp-AvrM gene.The bioinformatics analysis and its protein 3d structure analysis was carried out for Mlp-AvrM gene.Vaccination 2hpi rust hyphae entered through stomata to infect the leaves.Lots of haustoria formed during 24-48 hpi,infection hyphae intensively grew during 48-96 hpi,a complete life cycle was finished till168 hpi a complete life,and a new generation of urediospore was produced finally.A CDS of cloned Mlp-AvrM gene was 1242 bp,containing four exons and three introns in total,which encoded 413 amino acids.Protein structure analysis showed that the N-terminal contain an L structure constructed with an α-helices and a loop,and the C-terminal coiled domains of the Mlp-AvrM protein plays an important scaffolding role.关键词
Mlp-AvrM/效应因子/基因克隆/落叶松-杨栅锈菌Key words
Mlp-AvrM/effect factor gene/gene cloning/Melampsora larici-populina分类
农业科技引用本文复制引用
零雅茗,贾岩岩,李丹蕾,陈俏丽,王峰,张瑞芝,李永航..落叶松-杨栅锈菌效应因子Mlp-AvrM基因克隆[J].森林工程,2017,33(2):41-44,4.基金项目
国家自然科学基金项目(31500524) （31500524）
中央高校基本科研业务费专项资金项目(2572015EY02 （）
2572015CA09 （）
2572016CA04) （）
