色谱2017，Vol.35Issue(4)：405-412,8.DOI:10.3724/SP.J.1123.2016.10034
超高效液相色谱-四极杆/静电场轨道阱高分辨质谱检测食品中的牛奶过敏原酪蛋白
Determination of milk allergen caseins in foods by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry
摘要
Abstract
Based on ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q/Orbitrap MS) system, a rapid screening and quantitative detection method for milk allergen caseins was developed.After the sample was extracted with protein extraction buffer, a 5 kD ultrafiltration membrane was used to remove small molecule impurities.The extracted protein was then digested by trypsin.The full-scan mass spectra obtained by the data-dependent acquisition (DDA) mode were used for protein identification, and parallel reaction monitoring (PRM) technique was used for quantitative analysis of the target signature peptide.The internal standard peptide and the internal standard substances for every signature peptide were designed and synthesized to reduce the matrix effect and eliminate the loss in sample pretreatment.The proposed method showed a good linear relationship in the range of 5-250 μg/L.The limits of quantitation were in the range of 0.2-5.5 μg/kg.The observed recoveries ranged from 68.8% to 104.4%, and the RSDs were lower than 6%.This method can be used for rapid screening and quantitative analysis of milk allergen casein in juice, jam, bread and breakfast cereals.关键词
超高效液相色谱/四极杆/静电场轨道阱高分辨质谱/酪蛋白/食品Key words
ultra performance liquid chromatography (UPLC)/quadrupole/electrostatic field orbitrap high resolution mass spectrometry (Q/Orbitrap MS)/casein/foods分类
化学化工引用本文复制引用
詹丽娜,陈沁,古淑青,邓晓军..超高效液相色谱-四极杆/静电场轨道阱高分辨质谱检测食品中的牛奶过敏原酪蛋白[J].色谱,2017,35(4):405-412,8.基金项目
上海市自然科学基金项目(14ZR1450000) （14ZR1450000）
长三角科技联合攻关领域项目(15395810101,16395810101) （15395810101,16395810101）
国家质检总局科技项目(2016IK222).Shanghai Natural Science Foundation (No.14ZR1450000) （2016IK222）
Science and Technology Joint Project of the Yangtze River Delta (Nos.15395810101, 16395810101) （Nos.15395810101, 16395810101）
Scientific and Technological Project of the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (No.2016IK222). （No.2016IK222）
