食品工业科技2016，Vol.37Issue(11)：156-160,164,6.DOI:10.13386/j.issn1002-0306.2016.11.024
巨大芽孢杆菌NCT-2冻干菌剂的制备及冻干保护剂响应面优化
Preparation of Bacillus megaterium NCT-2 freeze-dried agent by using response surface methodology
摘要
Abstract
Vacuum freeze-drying method was used to prepare B.megaterium NCT-2 agents,cryoprotectants were optimized using response surface methodology after single factor experiment.Centrifugal conditions,suspension substrate,selection and formulation of cryoprotectants,rehydration water were studied on the impact of survival rate.The results showed that the optimal conditions were 5000 r/min,10 min and fresh media was the better choice as the suspended substrate.Different kinds of water did not had obvious impact on the survival rate after rehydration.Results indicated the optimal amounts of cryoprotectants formula were sucrose 4.51 mg/g,trehalose 0.9 mg/g,glucose 9.60 mg/g.The proven survival rate was 91.8％.关键词
巨大芽孢杆菌/真空冷冻干燥/保护剂/响应面优化Key words
Bacillus megaterium NCT-2/Vacuum freeze-drying/cryoprotectant/response surface methodology分类
轻工纺织引用本文复制引用
王大欣,张丹,初少华,支月娥,周培..巨大芽孢杆菌NCT-2冻干菌剂的制备及冻干保护剂响应面优化[J].食品工业科技,2016,37(11):156-160,164,6.基金项目
国家高技术研究发展计划(863计划)课题(2012AA101405). （863计划）
