生物技术通报2017，Vol.33Issue(4)：78-82,5.DOI:10.13560/j.cnki.biotech.bull.1985.2017.04.010
核酸适配体的体外筛选方法的最新研究进展
The Latest Progress on the Methods for in Vitro Screening Aptamers
摘要
Abstract
Aptamers are single-stranded oligonucleotides that are screened by systematic evolution of ligands by exponential enrichment (SELEX)in vitro,which can selectively bind to different target with high affinity and high specificity,including protein,small molecules, organic compound,metal ions,drugs,etc. This technology has attracted more attentions for its advantages and thus it has been applied widely in many aspects such as biological sensor,gene chip,new drug development,nano technology,etc. However,the traditional SELEX method is cumbersome,which usually takes several months to screen out the target of nucleic acid in high specificity. With the rapid development of SELEX,many novel screening methods have emerged in recent years. The screening cycle and the screening efficiency are improved with these new methods,and the application of aptamers is expanded. This review introduces several new screening methods in recent three years, including multiple GO-SELEX,SWCNTs-assisted cell-SELEX,on-chip Cell-SELEX,Sequence-constructive SELEX and High-Fidelity (HI FI)SELEX,It is helpful for us to know more about the latest progress on the methods for in vitro screening aptamers and to promote the application of aptamers in various fields..关键词
核酸适配体/SELEX/筛选方法Key words
aptamer/SELEX/screening method引用本文复制引用
李亚楠,赵洁,张傲哲,谭琰,华茜,张子剑..核酸适配体的体外筛选方法的最新研究进展[J].生物技术通报,2017,33(4):78-82,5.基金项目
国家自然科学基金项目(81403180) （81403180）
