生物技术通报2017，Vol.33Issue(4)：114-118,5.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0250
CP4-EPSPS转基因棉花植株鉴定方法比较分析
Comparative Analysis of Identification Methods of CP4-EPSPS Transgenic Cotton Plants
摘要
Abstract
CP4-EPSPS isolated from Agrobacterium strain CP4 is a glyphosate resistant gene and one of the most commonly used marker genes in transgenic plant research. However,we found that investigated results of the transgenic plants were not always completely accurate only using simple glyphosate resistance assay in the identification of CP4-EPSPS transgenic plants,which resulted in false-positive plants or missing positive transgenic plants. In this study,by use CP4-EPSPS transgenic cottons that we had gained,the four screening methods of CP4-EPSPS transgenic plants were compared,which included PCR,ELISA,resistant test strips and glyphosate resistance assay. The results show that the accuracy of ELISA and test strip are significantly higher than PCR and 0.3% glyphosate,but the test strip test is more convenient than ELISA basing on the comprehensive analysis,accuracy,timeliness,ease of use and price in identification of transgenic plants. Therefore we think combination glyphosate resistance assay with resistant strip test is the best choice. Our finding provides significant guidance for people engaging in the identification of CP4-EPSPS transgenic plants both in the laboratory and field.关键词
转基因棉花/CP4-EPSPS/草甘膦抗性/转基因植株筛选Key words
transgenic cotton/CP4-EPSPS/glyphosate resistance/identification of transgenic plants引用本文复制引用
郭文芳,王楠,李刚强,许芳芳,杨彩峰,刘德虎..CP4-EPSPS转基因棉花植株鉴定方法比较分析[J].生物技术通报,2017,33(4):114-118,5.基金项目
国家转基因生物新品种培育科技重大专项资助项目(2016ZX08005-004) （2016ZX08005-004）
