生物技术通报2017，Vol.33Issue(4)：143-148,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017.04.018
保护酶PPO在木薯种质抗螨中的功能初步研究
Preliminary Study on the Function of Polyphenol Oxidase in Cassava Resistance to Mite
摘要
Abstract
So far,the function of polyphenol oxidase(PPO)in cassava resistance to mite has not been understood clearly. In order to validate this function,we used quantitative real-time PCR(qPCR)and enzymatic assay to determine the changes of expression and activities of PPO in either cassava or Tetranychus cinnabarinus after the mite-resistant and mite-susceptible cassava cultivars were damaged by T. cinnabarinus. The results showed that,in one hand,compared to those in the same leaves before damaged,the transcriptions of MePPO and total activities of PPO only increased to 0.99-,1.02-fold and 1.05-,1.03-fold while the mite-susceptible cassava cultivar BRA900 was damaged by T. cinnabarinus for 1 d and 8 d,respectively. However,the corresponding values in 1 d- and 8 d-damaged leaves of mite-resistant cassava C1115 increased to 1.78-,1.74-fold and 1.74-,1.72-fold,respectively,thus significantly higher than those of mite-susceptible cassava. In the other hand,compared with those before feeding,the expressions of TcPPO and activities of PPO in T. cinnabarinus were 0.98-,0.97-fold and 0.98-,1.10-fold,respectively after feeding on BRA900 for 1 d and 8 d ;while the corresponding values in T. cinnabarinus feeding on C1115 decreased to 0.52-,0.64-fold and 0.54-,0.57-fold,respectively. This study preliminarily validated the function of PPO in cassava resistance to T. cinnabarinus.关键词
PPO/朱砂叶螨/木薯种质/抗螨功能Key words
polyphenol oxidase/Tetranychus cinnabarinus/cassava/resistance to mite引用本文复制引用
梁晓,卢芙萍,卢辉,伍春玲,曹宪红,陈青..保护酶PPO在木薯种质抗螨中的功能初步研究[J].生物技术通报,2017,33(4):143-148,6.基金项目
国家木薯产业技术体系害虫防控岗位科学家专项(CAR S-12-hncq)(CARS-12-hncq) （CAR S-12-hncq）
