生物技术通报2017，Vol.33Issue(4)：164-168,5.DOI:10.13560/j.cnki.biotech.bull.1985.2017.04.021
纳米碳对离体培养条件下几种植物生长及分化的影响
Effects of Nano Carbon on the Growth and Differentiation of Several Plants in Vitro Culture
摘要
Abstract
This work is to study the effects of different concentrations of nano carbon on growth and differentiation of several plants' explants. The test tube seedling of edible lily,Anthurium andraeanum,Cymbidium hybridum,and Jinchang jujube as experimental material was cultured on MS medium with different concentrations of nano carbon. Results showed that,MS + 6-BA 0.8 mg/L + NAA 0.2 mg/L +nano carbon 0.10 g/L was conducive to the multiplication of lily bulb with the multiplication coefficient 2.11 ;when the concentration of nano carbon was 0.50 g/L,the growth and rooting of lily in vitro was significantly better. The optimal medium for induction and differentiation of Anthurium's axillary buds was MS+ 6-BA 1.5 mg/L + IBA 0.2 mg/L + nano carbon 0.04 to 0.06 g/L,the induction rate was over 90% and the differentiation rate was 97%. The addition of nano carbon 1.0 to 2.0 g/L obviously inhibited the browning of C. hybridum protocorm subculture. The optimal medium for the multiplication cultivation of Jinchang jujube was MS + 6-BA 1.5 mg/L + IBA 0.5 mg/L + nano carbon 0.05 g/L,the multiplication coefficient reached 9.76 ;bud growth was robust when the concentration of nano carbon was 0.15 g/L.关键词
纳米碳/组织培养/增殖/褐化Key words
nano carbon/tissue culture/multiplication/browning引用本文复制引用
冯璐,王玉国,温银元,赵娟,刘圆,任建宏,贺美林..纳米碳对离体培养条件下几种植物生长及分化的影响[J].生物技术通报,2017,33(4):164-168,5.基金项目
山西省财政厅科技成果转化项目(K4714197) （K4714197）
