生物技术通报2017，Vol.33Issue(4)：185-190,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017.04.024
苏云金芽胞杆菌cry2Ab34基因的克隆、表达和杀虫活性分析
Cloning,Expression,and Insecticidal Activities of Gene cry2Ab34 from Bacillus thuringiensis
摘要
Abstract
In order to identify and detect insecticidal activities from Bacillus thuringiensis strain,the genomic DNA was extracted from the strain Bt BJH500 with high insecticidal activity,from which the insecticidal gene cry2Ab34(GenBank accession number :KX357382) was cloned by PCR. The gene was designated as cry2Ab34 by International Gene Nomenclature Committee of Bt. Gene cry2Ab34 was expressed and the weight of expressed product was approximate 70 kD. The gene was expressed in Escherichia coli BL21 for the determination of biological activity,results showed that this gene presented certain insecticidal activity to Plutella xyllostella and Helicoverpa armigera of Lepidoptera pests,the corrected mortality of 10 μg/mL was 31.25%,of 100 μg/mL was 62.5 %,of 10 μg/mL was 29.4 %,and of 100 μg/mL was 52.9 %.关键词
苏云金芽胞杆菌/cry2Ab34基因/杀虫晶体蛋白/杀虫活性/小菜蛾/棉铃虫Key words
Bacillus thuringiensis/gene cry2Ab34/insecticidal crystal protein/insecticidal activity/Plutella xyllostella/Helicoverpa armigera引用本文复制引用
张月,李海涛,刘荣梅,高继国..苏云金芽胞杆菌cry2Ab34基因的克隆、表达和杀虫活性分析[J].生物技术通报,2017,33(4):185-190,6.基金项目
转基因生物新品种培育科技重大专项(2014ZX0800913B-002),国家基础科学人才培养基金资助项目(J1210069),国家自然科学基金项目(31401812),黑龙江省教育厅科学技术研究项目(12521021) （2014ZX0800913B-002）
