生物技术通报2017，Vol.33Issue(4)：205-213,9.DOI:10.13560/j.cnki.biotech.bull.1985.2017.04.027
光电子响应微生物的筛选鉴定及生长代谢特征研究
Screening of Photoelectron-response Microbes as well as Their Growth and Metabolism
摘要
Abstract
Screening microbial strains that have promising response to semiconductor photoelectron brings broad application prospects in the study of interaction between minerals and microorganisms. Using the uranium tailing area soil,the microbial strains with photoelectron-response were screened in the media of containing 1/2,1/4,1/8,1/10,1/50,1/100,and 1/1000 semiconductor material TiN under illumination condition. Then the strain was identified through its morphological,physiological and biochemical characteristics,and the molecular phylogeny. Ultraviolet-visible absorption spectra and three-dimensional fluorescence spectra were used to analyze the growth of the strain and the changes of protein,humic acid,and other metabolites. The strain Y-5 screened from soil was identified to be Alcaligenes faecalis,which had strong response to photoelectron of TiN. The optoelectronic effect was not obvious due to rich nutrient in the 1/10 substrate concentration ;but in 1/50 and 1/100 low substrate concentration,the growth rate was accelerated by photoelectron due to poor nutrient,and the protein and humic acid increased correspondingly. A photoelectron-response microbial strain A. faecalis was screened from an uranium tailing area soil,and the photoelectron promoted its growth and metabolism. This method demonstrated certain feasibility in screening the photoelectron-response.关键词
光电子/粪产碱杆菌/生长代谢/蛋白质/腐殖酸Key words
photoelectron/Alcaligenes faecalis/growth and metabolism/protein/humic acid引用本文复制引用
向沙,刘明学,张格格,罗浪,魏红福,董发勤..光电子响应微生物的筛选鉴定及生长代谢特征研究[J].生物技术通报,2017,33(4):205-213,9.基金项目
国家重点基础研究发展计划("973")项目(2014CB846003),国家自然科学基金项目(41272371) （"973"）
